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Caption Schematic of the Gt(ROSA)26Sortm1(NOTCH3)Sat, Gt(ROSA)26Sortm2(NOTCH3*C455R)Sat and Gt(ROSA)26Sortm3(NOTCH3*R1031C)Sat constructs. Schematic representation of the targeting vectors used to generate conditional knock-in mice expressing human Notch 3 wild-type (hNOTCH3 WT; Gt(ROSA)26Sortm1(NOTCH3)Sat), or the C455R (Gt(ROSA)26Sortm2(NOTCH3*C455R)Sat) or R1031C (Gt(ROSA)26Sortm3(NOTCH3*R1031C)Sat) mutant receptors. Each construct contains genomic sequences allowing for homologous recombination into the ROSA locus; an adenovirus splice acceptor (SA); a PGK-neo-tpA "stop" cassette flanked by loxP sites (black triangles) allowing for Cre-mediated excision; the transgene; and the bovine growth hormone polyadenylation sequence (bpA). The NOTCH 3 WT and mutant transgenes are followed by an internal ribosomal entry site (IRES) and nuclear EGFP (nEGFP).
Copyright This image is from Arboleda-Velasquez JF, Proc Natl Acad Sci U S A 2011 May 24;108(21):E128-35. Copyright 2011 National Academy of Sciences, U.S.A. J:171887
Associated
Alleles
Symbol Name
Gt(ROSA)26Sortm1(NOTCH3)Sat gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Spyros Artavanis-Tsakonas
Gt(ROSA)26Sortm2(NOTCH3*C455R)Sat gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Spyros Artavanis-Tsakonas
Gt(ROSA)26Sortm3(NOTCH3*R1031C)Sat gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Spyros Artavanis-Tsakonas
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory