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| Caption | Generation of the L2hgdhGt(DC0625)Wtsi allele. We show the genomic structure of the L2hgdh gene in wild-type and mutant knock-out mice, which spans 10 exons (black boxes). In the disrupted allele, the gene trapping cassette [containing engrailed intron1 (En2 int1); Lox71 and LoxP Cre recombination sites (green and blue arrows); FRT flippase recombination sites (red arrows); Beta-geo cassette (orange box); splicing acceptor (SA) and polyadenylation (PA) sequences; a portion of pGTX1 vector (pUC backbone)] has been randomly inserted in intron 3, which leads to the production of an inactive protein comprising the first 137 residues of L-2-hydroxyglutarate dehydrogenase fused to the Beta-geo cassette. The latter confers resistance to neomycin and interrupts downstream transcription due to the addition of a polyadenylation signal. The precise site of insertion of the gene trapping cassette is shown, as well as the location of the primers (Fw-8; Rev-In3; Rev-TV) used for diagnostic amplification of the wild-type and mutated alleles. | ||||
| Copyright | This image is from Rzem R, PLoS One 2015;10(3):e0119540, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:228407 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/14/2024 MGI 6.23 |
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