L2hgdh^{Gt(DC0625)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: L2hgdh^{Gt(DC0625)Wtsi}
• Name: L-2-hydroxyglutarate dehydrogenase; gene trap DC0625, Wellcome Trust Sanger Institute
• MGI ID: MGI:3869865
• Gene: L2hgdh Location: Chr12:69737210-69771648 bp, - strand Genetic Position: Chr12, 28.94 cM
• Alliance: L2hgdh^{Gt(DC0625)Wtsi} page

Spongiotic appearance of the L2hgdh^{Gt(DC0625)Wtsi}/L2hgdh^{Gt(DC0625)Wtsi} brain

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: DC0625
• Germline Transmission: Earliest citation of germline transmission: J:228407
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pGT1lxr
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The gene trap vector inserted in the third intron, resulting in the production of a fusion transcript encoding the first 137 amino acid residues of L2hgdh followed by beta-geo. qPCR indicates low but detectable levels of transcripts indicating that the polyadenylation signal in the inactivation cassette is leaky. In addition, a small amount of readthrough transcript is synthesized and spliced as wild-type RNA. (J:228407)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  DC0625	DU132500 ; (MGI Seq Detail)	5' RACE	Chr12:69768085-69771619 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  4 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the L2hgdh^{Gt(DC0625)Wtsi} allele

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any L2hgdh Mutation: 17 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)