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| Caption | Generation of the Syne2tm1Chen allele. (A) Targeting strategy. A restriction map of the relevant genomic region of Nesprin 2 is shown at the top, the targeting construct is shown in the middle, and the mutated locus after recombination is shown at the bottom. The grey box indicates an exon which is the 16th exon, as counted backward from the last exon, triangular black boxes indicate LoxP sites and rectangular grey boxes indicate frt sites. DTA, Diphtheria Toxin A chain gene, Neo, Neomycin resistance gene. (B) Detection of wild-type (WT) and knockout (KO) alleles by Southern blot analysis. DNA from electroporated ES cells was digested with EcoR V and analyzed by Southern Blot analysis with a probe as shown in A. The 12.1 kb and 10.5 kb bands represent WT and KO alleles, respectively. (C) PCR analysis of DNA isolated from tails of Nesprin 2 WT, heterozygous and homozygous KO mice. KO and WT mice show only one band using specific primers for the KO or WT allele, heterozygous mice show two bands. (D) RT-PCR analysis with RNA from muscle. No coding sequence of the deleted exon could be amplified from the KO sample. (E and F) Immunostaining from (E) Cardiac Fibroblasts and (F) Cardiomyocytes (Blue = DAPI, Red = Nesprin 2) Green bar = 10 um, White Bar = 5 um. Green arrows indicate nuclear membranes (G) Real Time PCR analyses of Nesprin 2 knockout from isolated cardiomyocytes. student-t test #P<0.01. | ||||
| Copyright | This image is from Banerjee I, PLoS Genet 2014 Feb;10(2):e1004114, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:211007 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 01/20/2026 MGI 6.24 |
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