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| Caption | Generation of the Uso1Gt(YTA025)Byg (YTA025) and Uso1Gt(AW0562)Wtsi (AW0562) alleles. A) Schematic representation of the gene trap insertions into Uso1. The Uso1 exons upstream and downstream of the gene trap (GT) are shaded grey. These are exons 10 and 11 for AW0562 and exons 12 and 13 for YTA025. The GT contains intron 1 and a strong splice acceptor (SA) from the engrailed locus (En2-intron1-SA), coding sequence for Beta-Geo, and a poly-A (pA) addition site. Sites that can be used for Cre-mediated (Lox71 and LoxP) and Flpe-mediated recombination (Frt) are also contained within the GT. Primer pairs used for RT-PCR and genotyping are numbered and color-coded and their approximate locations within the exon, intron, or GT vector are indicated. | ||||||
| Copyright | This image is from Kim S, PLoS One 2012;7(11):e50530, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195576 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/14/2024 MGI 6.23 |
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