Uso1^{Gt(AW0562)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Uso1^{Gt(AW0562)Wtsi}
• Name: USO1 vesicle docking factor; gene trap AW0562, Wellcome Trust Sanger Institute
• MGI ID: MGI:3857238
• Gene: Uso1 Location: Chr5:92285797-92350657 bp, + strand Genetic Position: Chr5, 46.05 cM, cytoband E3
• Alliance: Uso1^{Gt(AW0562)Wtsi} page

Dispersed Golgi architecture in Uso1^{Gt(YTA025)Byg}/Uso1^{Gt(YTA025)Byg} and Uso1^{Gt(AW0562)Wtsi}/Uso1^{Gt(AW0562)Wtsi} blastocysts

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: AW0562
• Germline Transmission: Earliest citation of germline transmission: J:195576
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The gene trap vector inserted 541 bp downstream of exon 10. Immunohistochemistry confirmed the lack of protein expression in blastocysts. (J:195576)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AW0562	CW989994 ; (MGI Seq Detail)	5' RACE	Chr5:92321852-92325433 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  21 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Uso1^{Gt(YTA025)Byg} and Uso1^{Gt(AW0562)Wtsi} alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Uso1 Mutation: 63 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)