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| Caption | Generation of the Slc6a13tm1.1Ncd and Slc6a13tm1.2Ncd alleles. A, gene targeting construct contained exons 4-8 of the Slc6a13 gene. A loxP sequence (triangle) was inserted into intron 4, and frt-PGKneo-frt-loxP cassette was inserted into intron 7. The lengths of the homologous arms and the floxed fragment are indicated below the construct. The double arrows indicate the positions of the two sets of nested primers used for ES cell screening. They were located outside the construct and inside the neo cassette. The neo cassette was removed via FLP mediated recombination and generates the Slc6a13tm1.1Ncd allele. The genotyping primers were indicated by black arrows below the After FLP excision allele and After Cre excision allele. After Cre excision which generates the Slc6a13tm1.2Ncd allele, the DNA encoding amino acid residues 160-277 were deleted. This region is essential for transport activity, and there will be no transport activity without it. Furthermore, the deletion causes the remaining sequences to be out of frame. | ||||||
| Copyright | This image is from Zhou Y, J Biol Chem 2012 Oct 12;287(42):35733-46 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:191581 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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