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| Caption | Generation of a conditional gene trap mouse line for Gnas (Gnasxl). (A) The targeting construct comprises the Gnas exon 2 splice acceptor (Ex2SA) fused in frame to LacZ-pA, an frt-flanked neor cassette and pairs of loxP/lox2272 sites assembled in head-to-head orientation. The gene trap cassette was inserted in the non-functional (antisense) orientation at the rarely used A20 exon position. The neor cassette was removed via Flp-mediated recombination generating the Gnastm1.1Plag allele. Cre recombination results in inversion at compatible lox sites followed by excision between head-to-tail sites, which results in a stably integrated gene trap that attracts splicing from Gnas exon 1 and generates the Gnastm1.2Plag allele. The resulting XL domain - beta-galactosidase (XL-betaGal) fusion protein lacks XLas function, but retains beta-galactosidase activity. Relative positions of probes and restriction sites used in Southern-blots are shown, as are PCR primers (arrows) used in genotyping. | ||||||
| Copyright | This image is from Krechowec SO, PLoS One 2012;7(1):e29753, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:184320 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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