Gnas^tm1.2Plag
Targeted Allele Detail

Summary

• Symbol: Gnas^tm1.2Plag
• Name: GNAS complex locus; targeted mutation 1.2, Antonius Plagge
• MGI ID: MGI:5320901
• Gene: Gnas Location: Chr2:174126113-174188537 bp, + strand Genetic Position: Chr2, 97.89 cM, cytoband E1-H3
• Alliance: Gnas^tm1.2Plag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184320
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Cre-meditated recombination inverted the exon 2SA and lacZ leaving a lox2272 site at the 5' end and loxP site at the 3' end. The allele expresses a fusion product between the XL domain and lacZ. qRT-PCR confirmed a 90% downregulation of transcript expression. (J:184320)

Generation of the Gnas^tm1.1Plag and Gnas^tm1.2Plag alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnas Mutation: 66 strains or lines available

References

• Original: J:184320 Krechowec SO, et al., Postnatal changes in the expression pattern of the imprinted signalling protein XLalphas underlie the changing phenotype of deficient mice. PLoS One. 2012;7(1):e29753
• All: 1 reference(s)