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| Caption | (A) Insertion of the lox-Stop-lox-mito-YFP transgene (1) in the Gt(ROSA)26Sor locus (2) to generate the Gt(ROSA)26Sortm1Lrsn allele (3) and removal of the stop cassette to generate the Gt(ROSA)26Sortm1.1Lrsn allele (4). YFP fused to a mitochondrial targeting sequence (MTS) is placed downstream of a stop cassette, which contains tandem poly-adenylation signals (pA), a Westphal stop cassette (Stop), and a neomycin resistance gene (Neo), flanked by heterologous loxP-sites (lox2372). The construct is inserted downstream of exon 1 in the ROSA26 locus by homologous recombination (3). The short and long homology arms are shown in blue and red, respectively. When the stop cassette is removed by cre mediated excision (4) and generates the Gt(ROSA)26Sortm1.1Lrsn allele, fusion between the noncoding ROSA26 exon 1 and an introduced splice acceptor (SA), allows transgene expression by the endogenous ROSA26 promoter. FRT, Flippase recognition target. | ||||||
| Copyright | This image is from Sterky FH, Proc Natl Acad Sci U S A 2011 Aug 2;108(31):12937-42. Copyright 2011 National Academy of Sciences, U.S.A. J:176022 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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