Phenotype Image Detail

Image

Caption

At E9.5, Gcn1^em1(IMPC)J/Gcn1^em1(IMPC)J (Gcn1-/-) placentas exhibit impaired chorioallantoic attachment and multiple labyrinth zone defects compared to controls. The Tfeb probe, overstained during color development, resulting in lighter artifact staining adjacent to the true, intensely dark signal. In the KO placentas, the Tfeb+ region fails to expand and Gcm1+ chorioallantoic branching is severely impaired. Impaired chorioallantoic attachment is observed in most KO conceptuses examined (KO #2, n = 3/4). Although allantoic mesoderm is present adjacent to the chorion, the attachment is weak and easily disrupted during dissection. In the conceptus with normal attachment (KO #1, n = 1/4), less allantoic mesoderm is observed, and the resulting interface fails to expand as widely as in controls.

Copyright

This image was contributed directly to MGI. Questions regarding this image or these data should be directed to Dr. Kimberly D. Tremblay at kdtrembl@umass.edu. J:374598

Associated Alleles

Symbol	Name
Gcn1^em1(IMPC)J	GCN1 activator of EIF2AK4; endonuclease-mediated mutation 1, Jackson

Associated Genotypes

Allelic Composition	Genetic Background
Gcn1^em1(IMPC)J/Gcn1^em1(IMPC)J	C57BL/6NJ-Gcn1^em1(IMPC)J/Mmjax