Phenotype Image Detail

Image

Caption

Morphometric analysis of aortic root lesions in atheroprone male and female mice with (Apoe^tm1Unc/Apoe^tm1Unc Tg(Cdh5-cre/ERT2)1Rha/0 Nck1^tm1Paw/Nck1^tm1Paw Nck2^tm3Paw/Nck2^tm3Paw) and without (Apoe^tm1Unc/Apoe^tm1Unc Tg(Cdh5-cre/ERT2)1Rha/0 Nck1^tm1Paw/Nck1^tm1Paw) endothelial-specific deletion of NCK2. Animals were fed a HFD for 8 or 16 weeks to assess atherosclerotic lesion initiation and progression, respectively. (A/D) Representative images of aortic roots stained with H&E (top) and Picrosirius Red (bottom) to assess lesion development and collagen deposition, respectively. The scale bar represents 500 um. (B/E) Pie charts showing percentage of lesion category in each experimental group. Lesions were classified as intima thickening (type I), fatty streaks (type II), intermediate lesion (type III), advanced atheroma (type IV), and fibroatheroma (type V). Numbers of animals/group are indicated in parentheses. (C/F) Box plots showing quantification of plaque area, ratio of collagen to plaque area, and ratio of collagen to aortic root wall area. Statistical significance was determined by Wilcoxon Rank Sum Test with Bonferroni correction. *p < 0.05.

Copyright

This image is from the Laboratory of Dr. Gonzalo M Rivera at Texas A&M University and is displayed with the permission of the author. J:327349

Associated Alleles

Symbol	Name
Apoe^tm1Unc	apolipoprotein E; targeted mutation 1, University of North Carolina
Nck1^tm1Paw	non-catalytic region of tyrosine kinase adaptor protein 1; targeted mutation 1, Tony Pawson
Nck2^tm3Paw	non-catalytic region of tyrosine kinase adaptor protein 2; targeted mutation 3, Tony Pawson
Tg(Cdh5-cre/ERT2)1Rha	transgene insertion 1, Ralf H Adams

Associated Genotypes

Allelic Composition	Genetic Background
Apoe^tm1Unc/Apoe^tm1Unc Nck1^tm1Paw/Nck1^tm1Paw Nck2^tm3Paw/Nck2^tm3Paw Tg(Cdh5-cre/ERT2)1Rha/0	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6