Phenotype Image Detail

Image

Caption

Deletion and mutation of Gjc2 (Cx47) results in disturbed myelin basic protein (MBP) expression accompanied by astrogliosis and microglial activation in juvenile mice. A, D, G and J, Immunohistochemical analysis on 25 um sagittal brain slices obtained from 10-day-old animals revealed fewer myelinated, MBP-positive fine fibers pervading the cerebellar granular layer of Gjc2^tm2.1Kwi/Gjc2⁺ (Cx47^+/M282T), Gjc2^tm2.1Kwi/Gjc2^tm2.1Kwi (Cx47^M282T/M282T) and Gjc2^tm1(EGFP)Kwi/Gjc2^tm1(EGFP)Kwi (Cx47^-/-) mice compared to wild-type (Cx47^+/+) littermates. B, E, H and K, Delayed myelin formation is accompanied by elevated GFAP expression, indicating astrogliosis in the cerebellar white matter which appeared minor in Cx47^+/M282T tissue but was pronounced in cerebella of P10 Cx47^M282T/M282T and Cx47^-/- mice. Arrows indicate the transition of white matter to the granule cell layer. Compared to wild-type tissue fewer GFAP-positive cells were localized in the granular layer of Cx47^M282T/M282 cerebella. Note the GFAP-positive radial processes of Bergmann glia cells pervading the molecular layer. Signals of GFAP-positive Begmann glia processes were used as internal control for staining intensities. C, F, I and L, Microglia were constantly distributed throughout the whole brain. Activated microglia displayed increased levels of Iba1. In P10 cerebella microglia with elevated Iba1 expression were found to a greater extent in brain slices obtained from Cx47^+/M282T, Cx47^M282T/M282T and Cx47^-/- animals relative to wild-type tissue. Auxiliary lines (MBP and Iba1 stainings) indicate the granular and Purkinje layer transition. Scale bars: A-L, 200 um; insets, 50 um.

Copyright

This image is from Tress O, PLoS Genet 2011 Jul;7(7):e1002146, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:174197

Associated Alleles

Symbol	Name
Gjc2^tm1(EGFP)Kwi	gap junction protein, gamma 2; targeted mutation 1, Klaus Willecke
Gjc2^tm2.1Kwi	gap junction protein, gamma 2; targeted mutation 2.1, Klaus Willecke

Associated Genotypes

Allelic Composition	Genetic Background
Gjc2^tm1(EGFP)Kwi/Gjc2^tm1(EGFP)Kwi	involves: 129P2/OlaHsd * C57BL/6
Gjc2^tm2.1Kwi/Gjc2^tm2.1Kwi	involves: C57BL/6 * SJL
Gjc2^tm2.1Kwi/Gjc2^+	involves: C57BL/6 * SJL