Gene Expression Image Detail

Reference

Figure

ID

J:240319 Stauber M, et al., Dev Biol. 2017 Mar 15;423(2):170-188

4

MGI:6156363

Image

Caption

(A) Schematic view of a headfold stage mouse embryo (~E7.75; top) and of wildtype (wt), Noto-/- and Foxj1-/- mono-ciliated nodal cells (bottom). Cilia (green lines) in both Noto-/- and Foxj1-/- nodes are short and irregular and abnormally anchored to the cell cortex. Wildtype and residual Foxj1 expression is depicted by dark and light purple, respectively. The part of the embryo used in the screen is boxed in red. (B) Whole mount in situ hybridization on wildtype (a-d) and Noto null (e-h) embryos at early to late headfold stage (~E7.75-E8.0); a-h, view onto ventral node at the center of the images (cranial to the left); a', e', lateral view of node (arrow; dorsal view on same embryos as in a, e). (D) RT-PCR. M, DNA ladder; (-) control, cDNA synthesis without RNA; ~, 1700; H2O, PCR without primers as negative control; Hprt, PCR on a house keeping gene as positive control for the integrity of cDNA.

Copyright

Reprinted with permission from Elsevier from 10.1016/j.ydbio.2016.11.019 Dev Biol 423: 170-188, Stauber M; Weidemann M; Dittrich-Breiholz O; Lobschat K; Alten L; Mai M; Beckers A; Kracht M; Gossler A, Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse. Copyright 2017

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
Ba	MGI:6156813 (1700024G13Rik)
Ba'	MGI:6156813 (1700024G13Rik)
Bb	MGI:6156834 (1700012B09Rik)
Bc	MGI:6156840 (Cfap144)
Bd	MGI:6156860 (Plet1)
Be	MGI:6156813 (1700024G13Rik)
Be'	MGI:6156813 (1700024G13Rik)
Bf	MGI:6156834 (1700012B09Rik)
Bg	MGI:6156840 (Cfap144)
Bh	MGI:6156860 (Plet1)
D Foxj1	MGI:6156878 (Foxj1)
D 1700012B09Rik	MGI:6156881 (1700012B09Rik)
D 1700024G13Rik	MGI:6156888 (1700024G13Rik)
D 1700026L06Rik	MGI:6156892 (Spaca9)
D 1700123K08Rik	MGI:6156906 (1700123K08Rik)
D Agtrap	MGI:6156907 (Agtrap)
D Cacna1s	MGI:6157745 (Cacna1s)
D Casp4	MGI:6156958 (Casp4)
D Dusp16	MGI:6156960 (Dusp16)
D Fam183b	MGI:6156962 (Cfap144)
D Hdc	MGI:6156966 (Hdc)
D Mt1	MGI:6156969 (Mt1)
D Mt2	MGI:6156973 (Mt2)
D Plet1	MGI:6156975 (Plet1)
D Xdh	MGI:6156977 (Xdh)