Phenotype Image Detail

Image

Caption

(A) Confocal images of GM130, GRASP55 and DAPI staining of seminiferous tubule sections of P35 wild-type (WT) and Gorasp2^tm1.1Maal/Gorasp2^tm1.1Maal (Gorasp2-/-) mice at stage II-III, V, VIII and XII. Arrowheads indicate less compact and more diffuse Golgi in spermatocytes from mutant mice as compared to littermate controls. Scale bar, 10 um. (B) Pseudo-colored images of GM130 staining as a function of Golgi area. The threshold value was set to 5um² based on analysis of Golgi area distribution in function of seminiferous tubule stages. The automatic pipeline for the functions was encoded in Matlab. Representative pseudo-colored images for indicated tubule stages and genotypes are shown. Arrowheads indicate Golgi with area greater than 5 um². Note that Golgi complexes with large areas are essentially localized in the periphery of seminiferous tubules. Scale bar, 50 um. (C) Graph showing the percentages of Golgi with areas greater than 5um² in function of seminiferous tubule differentiation stages. Images used for quantitative analysis were obtained from three independent samples and the number of tubules analyzed was as follow: II-III, n = 27; IV, n = 8; VI-VIII, n = 37; IX, n = 16; X-XII, n = 41. ***: p <0.001. (D) Confocal images of GM130, SYCP3 and DAPI staining of seminiferous tubule sections of P35 WT and mutant mice at stage II-III. Scale bar, 50 um. Inserts: High magnification pictures highlighting GM130 staining in pachytene spermatocytes expressing SYCP3.

Copyright

This image is from Cartier-Michaud A, PLoS Genet 2017 Jun;13(6):e1006803, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:241919

Associated Alleles

Symbol	Name
Gorasp2^tm1.1Maal	golgi reassembly stacking protein 2; targeted mutation 1.1, Michel Aurrand-Lions

Associated Genotypes

Allelic Composition	Genetic Background
Gorasp2^tm1.1Maal/Gorasp2^tm1.1Maal	B6J.129S2(Cg)-Gorasp2^tm1.1Maal