Phenotype Image Detail

Image

Caption

The Axin2^canp/Axin2^canp gastrulation phenotype. (A-D) Expression of markers of the primitive streak and nascent mesoderm was reduced in E7.5 Axin2^canp/Axin2^canp (canp) embryos. Expression of the primitive streak marker T was reduced in mutant embryos that showed an accumulation of cells in the amniotic cavity (arrow in C) (C). Expression of the nascent mesoderm marker Tbx6 was reduced in all mutant embryos and absent in those embryos with cells accumulated in the amniotic cavity (D; arrow shows accumulation of cells). (E-H) Defects in gastrulation in mutants, seen in transverse sections of E7.5 embryos. (E) Laminin (red) is expressed between the germ layers of wild-type E7.5 embryos, with a gap at the position of the epithelial-to-mesenchymal transition (EMT) at the primitive streak (arrow marks the gap in laminin at the primitive streak). (G) In strongly affected mutants, laminin expression was interrupted in a larger region, highlighting the increased width of the mutant streak. In wild-type embryos, E-cadherin (green) is down-regulated in the cells of the mesodermal wings that have undergone the EMT (arrowhead) (F). In strongly affected mutant embryos, the cells in the bulged primitive streak expressed E-cadherin, although they had mesenchymal morphology (arrow) (H). Despite the accumulation of cells at the primitive streak, E-cadherin^- mesodermal wings were present in the mutants (arrowheads). (Scale bar in E-H, 100 um.)

Copyright

This image is from Qian L, Proc Natl Acad Sci U S A 2011 May 24;108(21):8692-7. Copyright 2011 National Academy of Sciences, U.S.A. J:171894

Associated Alleles

Symbol	Name
Axin2^canp	axin 2; canopus

Associated Genotypes

Allelic Composition	Genetic Background
Axin2^canp/Axin2^canp	C3.B6-Axin2^canp