Phenotype Image Detail

Image

Caption

Pten^tm2Mak/Pten^tm2Mak Stk11^tm1Keis/Stk11^tm1Keis Tg(Cyp1a1-cre/ERT)1Dwi/0 (Pten/Lkb1^fl/fl) mouse urothelium undergoes epithelial-mesenchymal transition (EMT). Mouse bladder epithelium deficient for both Stk11 (Lkb1) and Pten exhibits loss in epithelial markers and acquire mesenchymal properties by day 100. Rapamycin inhibition of mTOR suppresses the observed effect. A, E-cadherin and ZO-1 immunofluorescent co-localization showing disappearance of these proteins from cell-cell walls in double mutant bladder whereas rapamycin treatment prevents this loss, B, Immunostaining showing a strong increase in Vimentin levels and Snail nuclear localisation after the combined deletion of Stk11 and Pten and this is mediated via mTOR evidenced from their suppressed levels in rapamycin-treated animals. Scale bars correspond to 20 um.

Copyright

This image is from Shorning BY, PLoS One 2011;6(1):e16209, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:169565

Associated Alleles

Symbol	Name
Pten^tm2Mak	phosphatase and tensin homolog; targeted mutation 2, Tak W Mak
Stk11^tm1Keis	serine/threonine kinase 11; targeted mutation 1, Kei Sakamoto
Tg(Cyp1a1-cre/ERT)1Dwi	transgene insertion 1, Douglas J Winton

Associated Genotypes

Allelic Composition	Genetic Background
Pten^tm2Mak/Pten^tm2Mak Stk11^tm1Keis/Stk11^tm1Keis Tg(Cyp1a1-cre/ERT)1Dwi/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6J * CBA