Phenotype Image Detail

Image

Caption

Loss of germ cells from Mad2l2^tm1.1Mpz/Mad2l2^tm1.1Mpz ovaries and testes. (B) Hematoxylin and Eosin (HE) staining of ovaries with low (upper panel) and high (lower panel) magnifications. Mutant ovaries (P80) are smaller, and do not contain follicular or germ cells. (C) Testes (P70) are significantly smaller in mutant animals. (D) Morphologic analysis of testes (upper panel) and epididymis (lower panel) by HE staining reveals the absence of germ cells in mutant organs (P70). (F-H) Mutant seminiferous tubules (P14) lack spermatogonial cells as identified by Plzf, pre-meiotic cells as identified by Stra8, and meiotic cells as identified by gammaH2AX. (I) Mutant seminiferous tubules (P70) contain highly vacuolated (red arrow) and miss-localized (arrowhead) Sertoli cells as identified by Wt1. Note hyperplasia of Leydig cells between seminiferous tubules (black arrow). Scale bars in B, E-I, 100 um, in D, 200 um.

Copyright

This image is from Pirouz M, PLoS Genet 2013 Aug;9(8):e1003712, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:223048

Associated Alleles

Symbol	Name
Mad2l2^tm1.1Mpz	MAD2 mitotic arrest deficient-like 2; targeted mutation 1.1, Mehdi Pirouz

Associated Genotypes

Allelic Composition	Genetic Background
Mad2l2^tm1.1Mpz/Mad2l2^tm1.1Mpz	involves: 129S2/SvPas * BALB/cJ