Phenotype Image Detail

Image

Caption

Early loss of FOG2-GATA4 interaction. A) At E12.5, Gata4^tm1.1Wtp/Gata4^tm1Sho Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ (Gata4^Ki-NK) embryos showed edema and peripheral hemorrhage. (B) Reduced coronary plexus in Gata4^tm1.1Wtp/Gata4^tm1Sho Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ mice, demonstrated by wholemount PECAM staining of E13.5 embryo hearts. n=3-5 per group. Bar=100 um. (C-D) H.E. staining of E14.5 embryos showed atrioventricular (AV) cushion defect, ventricular septal defect (VSD) (asterisk) and thin myocardium (white arrows). Bar=500 um. (E) Short axis adult heart sections stained with H.E. Gata4^tm1.1Wtp/Gata4^tm1Sho Tg(Myh6-cre)2182Mds/0 (Gata4^Ki-MC) mice had enlarged and dilated ventricles compared with controls. Bar=1 mm. (F) Fibrosis marker HSP47 staining was significantly increased in Gata4^tm1.1Wtp/Gata4^tm1Sho Tg(Myh6-cre)2182Mds/0 hearts compared with controls. Bar=50 um.

Copyright

This image is from Zhou B, J Clin Invest 2009 Jun;119(6):1462-76 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:150452

Associated Alleles

Symbol	Name
Gata4^tm1.1Wtp	GATA binding protein 4; targeted mutation 1.1, William T Pu
Gata4^tm1Sho	GATA binding protein 4; targeted mutation 1, Stuart Orkin
Nkx2-5^tm1(cre)Rjs	NK2 homeobox 5; targeted mutation 1, Robert J Schwartz
Tg(Myh6-cre)2182Mds	transgene insertion 2182, Michael D Schneider

Associated Genotypes

Allelic Composition	Genetic Background
Gata4^tm1Sho/Gata4^tm1.1Wtp Nkx2-5^tm1(cre)Rjs/Nkx2-5^+	involves: 129S6/SvEvTac * 129S7/SvEvBrd
Gata4^tm1Sho/Gata4^tm1.1Wtp Tg(Myh6-cre)2182Mds/0	involves: 129S6/SvEvTac * 129S7/SvEvBrd