Phenotype Image Detail

Image

Caption

Double stranded break (DSB) repair in Spo11^tm1.1Gvpc/Spo11^tm1.1Gvpc (Spo11^Gal/Gal)) spermatocytes is incomplete. Chromosomal spreads from earlier (zygotene) and later (pachytene) stage spermatocytes were immunostained with antibodies to gamma-H2AX (red) and SCP3 (green). Note the disappearance of gamma-H2AX staining at homologously synapsed autosomes in later stage wild-type (WT) and Spo11^tm1.1Gvpc/Spo11^tm1.1Gvpc spermatocytes consistent with proper repair of DSBs. gamma-H2AX persist in Spo11^tm1.1Gvpc/Spo11^tm3Rdco (Spo11^Gal/-) spermatocytes and on asynapsed chromosomes in Spo11^tm1.1Gvpc/Spo11^tm1.1Gvpc spermatocytes. Sex body (white arrow) is evident in WT, but not Spo11^tm1.1Gvpc/Spo11^tm1.1Gvpc spermatocytes.

Copyright

This image is from Smagulova F, BMC Genomics 2013;14():493, an open-access article, licensee BioMed Central Ltd. J:202231

Associated Alleles

Symbol	Name
Spo11^tm1.1Gvpc	SPO11 initiator of meiotic double stranded breaks; targeted mutation 1.1, Galina Petukhova
Spo11^tm3Rdco	SPO11 initiator of meiotic double stranded breaks; targeted mutation 3, R Daniel Camerini-Otero

Associated Genotypes

Allelic Composition	Genetic Background
Spo11^tm1.1Gvpc/Spo11^tm1.1Gvpc	involves: C57BL/6
Spo11^tm1.1Gvpc/Spo11^tm3Rdco	involves: C57BL/6