Phenotype Image Detail

Image

Caption

Electron microscopy reveals structural abnormalities in Ube2j1^tm1.2Hpl/Ube2j1^tm1.2Hpl (Ube2j1^-/-) spermatozoa. A, cross sections of a mutant stage IX testis shows retained spermatids (asterisks) with cytoplasm (arrows) above a layer of elongate spermatids. ES, ectoplasmic specialization. B, Wild-type (WT) elongated spermatids in the testis. C and D, Mutant and (E) WT spermatids in cross sectioned epididymides. F and G, Mutant and (H) WT spermatids isolated from epididymides. Mutant spermatozoa retain excess cytoplasm around the acrosome and neck region. Note vacuoles (wedges) surrounded by one or more membranes (F), and retained condensed ER (radial body, insets) in mutant spermatozoa (C, D, F, G). Scale bars, 2 um (A and B); 500 nm (C-F); 100 nm (G); 500 nm (H).

Copyright

This image is from Koenig PA, J Biol Chem 2014 Dec 12;289(50):34490-502 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:218292

Associated Alleles

Symbol	Name
Ube2j1^tm1.2Hpl	ubiquitin-conjugating enzyme E2J 1; targeted mutation 1.2, Hidde Ploegh

Associated Genotypes

Allelic Composition	Genetic Background
Ube2j1^tm1.2Hpl/Ube2j1^tm1.2Hpl	involves: BALB/cJ * C57BL/6 * C57BL/6N * SJL