Phenotype Image Detail

Image

Caption

Gng5^tm1.1Rbs/Gng5^tm1.1Rbs (Gng5-/-) mutants fail to form the cardiac outflow tract and right ventricle and have severely hypoplastic pharyngeal arches. Panels A-D images of E8.5 whole mount wild type (Gng5+/+, WT) and mutant embryos. By comparing the left lateral (A, C) and ventral (B, D) views of intact WT and mutant embryos, respectively, the unlooped heart tube is clearly evident (B, D). Panels E-H, images of E9.5 WT and mutant embryos. The left parasagittal section from a control embryo shows the inflow tract and left side of common atrium, endocardial cushion in the atrioventricular canal, and left ventricle (E). The red arrowheads mark the first pharyngeal arch, also labeled 1. The midline section shows the outflow tract connecting to aortic sac in the second pharyngeal arch (bracket), right ventricle, the right portion of the common atrium, and the sinus venosus in the control embryo (F). The left parasagittal section of the mutant shows severely hypoplastic but vascularized first pharyngeal arch (red arrowhead), dilated heart tube with atrial chamber caudal to ventricle, narrow inflow, and paucity of cells in the pharyngeal mesoderm (G). The midline section shows the unlooped, dilated heart tube and no outflow tract; cardiac chamber opens directly into dilated aortic sac (bracket) in a mutant embryo (H). TB, tail bud; HF, head fold; OFT, outflow tract; RV, right ventricle; LV, left ventricle; A, atrium V, ventricle; EC, endocardial cushion; SV, sinus venosus.

Copyright

This image is from Moon AM, PLoS One 2014;9(3):e90970, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:215128

Associated Alleles

Symbol	Name
Gng5^tm1.1Rbs	G protein subunit gamma 5; targeted mutation 1.1, Janet D Robishaw

Associated Genotypes

Allelic Composition	Genetic Background
Gng5^tm1.1Rbs/Gng5^tm1.1Rbs	B6J.Cg-Gng5^tm1.1Rbs