Phenotype Image Detail

Image

Caption

Control and Ptbp1^tm1Msol/Ptbp1^tm1Msol (KO) embryo sections at E7.5 immunolabelled for BrdU (top row) and processed by TUNEL (bottom row). The former is a cell division assay measuring the integration of the nucleotide analogue bromodeoxyuridine (BrdU). The latter is an apoptosis assay based on terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Control embryos (1st column) showed strong BrdU signal especially in the epiblast, the yolk sac area, and the ectoplacental cone while being negative for TUNEL. Heterozygous decidua were typically BrdU-labelled most strongly in the periphery, visible only in KO1 and cropped in control1 and KO2. Homozygous mutant embryos showed less (KO1) to no BrdU labelling (KO2) varying with embryo and section. Most null mutants analysed showed some degree of TUNEL (exemplified by KO3 and KO4). The longest diameter of the embryo is indicated below each panel and the relative sizes of the panels are indicated with dashed lines.

Copyright

This image is from Suckale J, PLoS One 2011;6(2):e16992, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:223915

Associated Alleles

Symbol	Name
Ptbp1^tm1Msol	polypyrimidine tract binding protein 1; targeted mutation 1, Michele Solimena

Associated Genotypes

Allelic Composition	Genetic Background
Ptbp1^tm1Msol/Ptbp1^tm1Msol	B6J.129-Ptbp1^tm1Msol