Phenotype Image Detail

Image

Caption

Sel1l^{Gt(CA0017)Wtsi}/Sel1l^{Gt(CA0017)Wtsi} (Sel1l-deficient) pancreatic epithelium exhibits impaired growth, branching morphology and differentiation ex vivo. Dorsal pancreatic buds of wild-type (WT) and mutant embryos were isolated at E11.5 and cultured. The genotypes of the pancreatic explants are indicated as "+/+" and "-/-", respectively. Cultured pancreatic explants were immunostained using the indicated antibodies. Mutant pancreatic epithelium grows poorly and displays impaired branching morphology (B and D), as compared to WT control explants (A and C). The number of insulin+ cells in mutant pancreatic epithelia were markedly reduced (B, arrows), as compared to the WT controls (A, arrows). Amylase expression was detected in mutant pancreatic epithelium (D, arrows), but the expression is significantly lower than that in WT epithelium (C, arrows). Scale bar: 100 um.

Copyright

This image is from Li S, BMC Dev Biol 2010;10():19, an open-access article, licensee BioMed Central Ltd. J:158780

Associated Alleles

Symbol	Name
Sel1l^Gt(CA0017)Wtsi	sel-1 suppressor of lin-12-like (C. elegans); gene trap CA0017, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Sel1l^Gt(CA0017)Wtsi/Sel1l^Gt(CA0017)Wtsi	involves: 129P2/OlaHsd