Phenotype Image Detail

Image

Caption

Osteoclast-intrinsic role of SLC4A2. (A) Photographs of 3-week-old mice. (B) Three-dimensional microquantitative computed tomography images of femurs from 8-week-old mice. (C) Bone volume per tissue volume (BV/TV) (means +/- SE) of the distal femoral metaphysis of 3- and 8-week-old mice (**P < 0.01, Slc4a2^tm2Ges/Slc4a2^tm2Ges Ctsk^tm1(cre)Ska/Ctsk⁺ (cKO) versus Slc4a2^tm2Ges/Slc4a2^tm2Ges (Slc4a2^fl/fl Ctsk-Cre-); similar results were obtained when cKO was compared with Slc4a2^tm2Ges/Slc4a2⁺ Ctsk^tm1(cre)Ska/Ctsk⁺ (Slc4a2^fl/+ Ctsk-Cre+). (D and E) Toluidine blue (D) and TRAP (E) stains of tibiae from 8-week-old mice. Images are representative of at least three sex- and age- matched littermate mice analyzed per genotype. (Scale bars, 500 um.) (F and G) Toluidine blue stain (F) and supernatant C-terminal type I collagen fragments (CTX) ELISA (G) (means +/- SE) of bone slices incubated with bone marrow macrophages and M-CSF and RANKL. (Scale bars, 200 um.) Representative of three separate experiments (*P < 0.05, **P < 0.01). (H) TRAP stain of osteoclast-like cells.

Copyright

This image is from Coury F, Proc Natl Acad Sci U S A 2013 Feb 5;110(6):2163-8. Copyright 2013 National Academy of Sciences, U.S.A. J:193823

Associated Alleles

Symbol	Name
Ctsk^tm1(cre)Ska	cathepsin K; targeted mutation 1, Shigeaki Kato
Slc4a2^tm2Ges	solute carrier family 4 (anion exchanger), member 2; targeted mutation 2, Gary E Shull

Associated Genotypes

Allelic Composition	Genetic Background
Slc4a2^tm2Ges/Slc4a2^tm2Ges Ctsk^tm1(cre)Ska/Ctsk^+	involves: C57BL/6NCrlj * CBA/JNCrlj