Phenotype Image Detail

Image

Caption

Loss of Cdc42 phenocopies the Yap1^tm1Hmc/Yap1^tm1Hmc Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺ phenotype. (A,B) Macroscopic view of the urogenital system from wild-type and Cdc42^tm1Brak/Cdc42^tm1Brak Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺ (Cdc42^CM-/-) kidneys at P0. Note the reduction in kidney bladder size in mutant animals. (C-F) PAS staining (P0) from wild-type and mutant animals showing smaller papilla (arrows), dramatic reduction of both CM-derived epithelial structures and glomeruli in the mutant. (G-J) Sections of P0 kidneys using late nephron-specific markers confirms the abnormal glomeruli and proximal tubules formation in mutant kidneys. Glomeruli (Podocin, G,H). Proximal tubules (LTL,I,J). (K,L) NCAM staining (E15.5) reveals dramatic reduction in the number of CM-derived structures (arrowheads) in mutants compared to wild-type. k: kidney; b: bladder; g: glomeruli; pt: proximal tubule. Scale bars represent 1 mm (A-D), 200 um (E-L).

Copyright

This image is from Reginensi A, PLoS Genet 2013 Mar;9(3):e1003380, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195266

Associated Alleles

Symbol	Name
Cdc42^tm1Brak	cell division cycle 42; targeted mutation 1, Cord Brakebusch
Six2^tm1(tTA,tetO-EGFP/cre)Amc	sine oculis-related homeobox 2; targeted mutation 1, Andrew P McMahon

Associated Genotypes

Allelic Composition	Genetic Background
Cdc42^tm1Brak/Cdc42^tm1Brak Six2^tm1(tTA,tetO-EGFP/cre)Amc/Six2^+	involves: 129 * C57BL/6J