Phenotype Image Detail

Image

Caption

Analysis of gastrulation defects in Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (cripto F78A/F78A) mutants reveals a hypomorphic phenotype. Wild-type (WT, top), Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A, middle), and Cripto^tm1Eda/Cripto^tm1Eda (KO, bottom) embryos at 7.5 dpc stained by whole-mount mRNA in situ hybridization. All embryos are shown with anterior side toward the left. (A, A' and A") Double staining of Brachyury (blue) and Otx2 (red) mRNAs in the primitive streak and anterior neuroectoderm, respectively, reveals normal A-P patterning in wild-type (A) and Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A) embryos (A'). In contrast, in Cripto^tm1Eda/Cripto^tm1Eda (KO) mutants (A"), cells expressing Brachyury and Otx2 remain in the proximal and distal epiblast, respectively. (B, B', and B") Double staining for Nodal (blue) and Otx2 (red) in wild-type embryos marks the node and the anterior neuroectoderm, respectively (B). In Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A) embryos (B'), Nodal mRNA is expressed in a group of posterior cells, whereas it is confined to the embryonic-extraembryonic boundary in Cripto^tm1Eda/Cripto^tm1Eda (KO) mutants (B"). (C, C', and C") Double staining of Foxa2 (blue) and Brachyury (red). (C) Foxa2 is expressed in the node and axial mesendoderm of wild-type embryos. (C') In Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A) embryos, Foxa2 marks both the distal primitive streak and the anterior side of the embryo. (C'') Foxa2 is not expressed in Cripto^tm1Eda/Cripto^tm1Eda (KO) embryos. (D, D', and D") Double staining for Cer-1 (blue) and Brachyury (red) reveals that Cer-1 marks the anterior definitive endoderm in both wild-type (D) and Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A) (D') embryos. In contrast, Cer-1 expression is absent in Cripto^tm1Eda/Cripto^tm1Eda (KO) mutants (D"). (E, E', and E") Double staining for Chordin (blue) and Brachyury (red). Chordin marks the axial mesendoderm in wild-type (E) and Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A) (E', arrowhead) embryos; however, the axial mesoderm is not expressed in Cripto^tm1Eda/Cripto^tm1Eda (KO) mutants (E'). (s1 and s2) Transverse sections taken from the embryo in C'. Foxa2 signal is localized throughout the anterior proximal region of the embryo, revealing the presence of mesoderm and definitive endoderm (s1). Brachyury expression is confined to posterior embryonic and extraembryonic mesoderm. Bars, 50 um.

Copyright

This image is from D'Andrea D, J Cell Biol 2008 Feb 11;180(3):597-605, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:186907

Associated Alleles

Symbol	Name
Cripto^tm1.1Gmin	cripto, EGF-CFC family member; targeted mutation 1.1, Gabriella Minchiotti
Cripto^tm1Eda	cripto, EGF-CFC family member; targeted mutation 1, Eileen D Adamson

Associated Genotypes

Allelic Composition	Genetic Background
Cripto^tm1.1Gmin/Cripto^tm1.1Gmin	involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Cripto^tm1Eda/Cripto^tm1Eda	either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * Black Swiss * C57BL/6)