Phenotype Image Detail

Image

Caption

Patterns of micromass stained by Alcian blue at day 3 (A and B), 6 (C and D), and 9 (E and F) of Tg(Prrx1-cre)1Cjt/0 (Prx1-Cre/Vcan+/+; control) (A, C, and E) and Vcan^tm1.1Hwat/Vcan^tm1.1Hwat Tg(Prrx1-cre)1Cjt/0 (Prx1-Cre/Vcan^flox/flox; mutant) (B, D, and F) are shown. At day 3, micromass of control contains some cartilaginous nodules stained with Alcian blue (A), whereas that of the mutant contains a smaller number of them (B). At day 6, the number of the nodules positive for Alcian blue in micromass of mutant increases, but it remains smaller than that of controls (C and D). At day 9, the number of cartilaginous nodules in micromass of control further increases, and the nodules in the center become strongly stained with Alcian blue (E). Although the number of cartilaginous nodules in micromass of mutant increases during culture (F), it remains smaller than controls. Scale bars, 300 um.

Copyright

This image is from Choocheep K, J Biol Chem 2010 Jul 2;285(27):21114-25 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:165936

Associated Alleles

Symbol	Name
Tg(Prrx1-cre)1Cjt	transgene insertion 1, Clifford J Tabin
Vcan^tm1.1Hwat	versican; targeted mutation 1.1, Hideto Watanabe

Associated Genotypes

Allelic Composition	Genetic Background
Vcan^tm1.1Hwat/Vcan^tm1.1Hwat Tg(Prrx1-cre)1Cjt/0	involves: C57BL/6 * C57BL/6J * SJL/J