Phenotype Image Detail

Image

Caption

Cdk1^tm2.1Kald/Cdk1^tm2.1Kald Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 (Cdk1^Liv-/-) hepatocytes do not enter mitosis after partial hepatectomy. Seventy percent of control (Cdk1^FLOX) and mutant livers were removed by partial hepatectomy to induce entry of hepatocytes into cell cycle. At 48 hours after partial hepatectomy, livers were isolated, and sections were stained with anti-phospho-histone H3 (Ser10) antibodies (06-570; Upstate Biotechnology) to monitor cells entering mitosis. Strongly stained mitotic cells are readily detectable in controls (Upper) but not mutant (Lower) liver sections. Insets in the Lower show spleen sections from the same mutant mice, and they were costained with the liver sections as a positive control. Two different animals were used for control and mutant sections.

Copyright

This image is from Diril MK, Proc Natl Acad Sci U S A 2012 Mar 6;109(10):3826-31. Copyright 2012 National Academy of Sciences, U.S.A. J:182141

Associated Alleles

Symbol	Name
Cdk1^tm2.1Kald	cyclin dependent kinase 1; targeted mutation 2.1, Philipp Kaldis
Speer6-ps1^Tg(Alb-cre)21Mgn	spermatogenesis associated glutamate (E)-rich protein 6, pseudogene 1; transgene insertion 21, Mark A Magnuson

Associated Genotypes

Allelic Composition	Genetic Background
Cdk1^tm2.1Kald/Cdk1^tm2.1Kald Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: 129 * C57BL/6 * DBA * SJL