Gene Expression Image Detail

Reference

Figure

ID

J:52200 Holmes GP, et al., Mech Dev. 1998 Dec;79(1-2):57-72

4

MGI:5444999

Image

Caption

(A) Expression in the floor plate (fp), floor (long arrow) and roof (small arrows) of the diencephalon (di). A patch of expression is also seen rostral to the optic stalk (os); te: telencephalon. (B) Expression in the floor plate (fp) up to the posterior end of the midbrain as marked (arrowhead). No expression was seen in the diencephalon (di) or telencephalon (te). Long arrow indicates expression in an area immediately adjacent to the floor plate at the level of Rhombomere 4. (C) Expression in floor plate (fp, long arrow), floor of diencephalon (dual arrowhead), roof of the diencephalon (small arrows), a small region rostral to optic stalk (os) and roof of midbrain and diencephalon (large arrowhead). Region of expression in ventral tongue marked by asterisk. (D) The region of the cross-section is depicted as a dotted line in (C). Expression is seen in the roof of the midbrain (arrowhead), floor plate (arrow) and floor of the diencephalon (dual arrowhead). (E) Dorsal view of a whole mount preparation of hindbrain at E13.5. Expression of Slit2 can be seen in the motor nuclei ofthe XII nerve (large arrowheads). Stronger expression of Slit2 can be seen in the floor plate toward the rostral end (long arrows). Two distinct cell groups onrhombomere 5 (asterisks) express Slit2 in a bilaterally symmetrical manner. A streak of staining connecting these cell groups to the midline were alsolabelled. (F, G) Thoracic cross-sectional views of whole mount preparations at E11.5 labelled with Slit2 (F) and Slit1 (G). (F) Slit2 expression is strong in thenotochord (nc), floor plate (fp), roof plate (rp) and motor neurons (mn). (G) Slit1 expression is also seen in the floor plate, roof plate (reduced in comparisonto Slit2) and motor neurons, but was absent from the notochord. (H) Cross-section of caudal neural tube at E9.5. Slit2 is only expressed in the notochord (nc)and roof plate (see Fig. 3B). (I) At E10.5, Slit2 is expressed in floor plate (fp), roof plate (rp) and developing motor neurons (mn), in addition to the notochord (nc). (J) E12.5 spinal cord cross-section. Note that pre-migratory motor neurons near the ventricular zone (asterisks) also express Slit2. (K) Ventral half ofspinal cord at E13.5. Slit2 is expressed in floor plate (fp) and somatic (smn) and autonomic preganglionic motor neurons (amn). (L) Cross-section of spinalcord at E16.5. Motor neurons (mn) and floor plate (fp) continue to express Slit2 mRNA; central canal (cc). Scale: 250 um (A,B), 500 um (C), 300 um (D,E),60 um (F,G), 50 um (H-J), 100 um (K,L). All panels were analysed using murine riboprobes.

Copyright

Reprinted with permission from Elsevier from 10.1016/S0925-4773(98)00174-9 Mech Dev 79: 57-72, Holmes GP; Negus K; Burridge L; Raman S; Algar E; Yamada T; Little MH, Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis. Copyright 1998

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A	MGI:5445094 (Slit2)
B	MGI:5445095 (Slit3)
C	MGI:5445094 (Slit2)
D	MGI:5445094 (Slit2)
E	MGI:5445094 (Slit2)
F	MGI:5445094 (Slit2)
G	MGI:5445095 (Slit3)
H	MGI:5445094 (Slit2)
I	MGI:5445094 (Slit2)
J	MGI:5445094 (Slit2)
K	MGI:5445094 (Slit2)
L	MGI:5445094 (Slit2)