Phenotype Image Detail

Image

Caption

Morphology and phenotype of bone marrow-derived cells from wild-type and Srgn^tm1Mabr/Srgn^tm1Mabr mice. Bone marrow cells were recovered from wild-type (+/+) and Srgn^tm1Mabr/Srgn^tm1Mabr (-/-) littermates and were cultured in the presence of WEHI-3B-conditioned medium as a source of IL-3. Cytospin slides were prepared at the time points indicated and were stained by May Grunwald/Giemsa. Note the empty vesicles present in both wild-type and mutant cells at time points up to 3 weeks (3w) of culture (black arrows). Note also that the empty vesicles are absent in wild-type cells after 5 weeks (5w) of culture and are replaced by the metachromatic granule (white arrow; not present in mutant cells). At 5 weeks, chloroacetate esterase activity was measured in situ (see insets).

Copyright

This image is from Abrink M, J Biol Chem 2004 Oct 24;279(39):40897-905 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:93338

Associated Alleles

Symbol	Name
Srgn^tm1Mabr	serglycin; targeted mutation 1, Magnus Abrink

Associated Genotypes

Allelic Composition	Genetic Background
Srgn^tm1Mabr/Srgn^tm1Mabr	involves: 129X1/SvJ