Phenotype Image Detail

Image

Caption

A and B: Acinar cells in the Eif2ak3^tm1.1Drc/Eif2ak3^tm1.1Drc Tg(Ela1-cre)16Eps/0 (exPKO) pancreas have numerous TUNEL-positive cells (A, P31 wild type; B, P22 Eif2ak3^tm1.1Drc/Eif2ak3^tm1.1Drc Tg(Ela1-cre)16Eps/0). Note that the TUNEL staining pattern strikingly differs between the two genotypes. TUNEL, green; DAPI, blue; background, red. C and D: Adjacent serial sections (3 microns) of the mutant pancreas (P19). Pale pink (oncotic) acinar cells, identified by hematoxylin and eosin staining (C) correspond to cells positive for ApoE (D); see arrows as examples of several cells. ApoE, red; DAPI, blue; background, green. E and F: The mutant pancreas has significant leukocyte infiltration (E, wild type; F, mutant, P74). Dark purple acid phosphatase staining indicated infiltrated leukocytes including macrophages (see arrows) in F but not E. The background cellular staining is methylene blue.

Copyright

This image is from Iida K, BMC Cell Biol 2007;8():38, an open-access article, licensee BioMed Central Ltd. J:129974

Associated Alleles

Symbol	Name
Eif2ak3^tm1.1Drc	eukaryotic translation initiation factor 2 alpha kinase 3; targeted mutation 1.1, Douglas R Cavener
Tg(Ela1-cre)16Eps	transgene insertion 16, Eric P Sandgren

Associated Genotypes

Allelic Composition	Genetic Background
Eif2ak3^tm1.1Drc/Eif2ak3^tm1.1Drc Tg(Ela1-cre)16Eps/0	involves: 129S6/SvEvTac