Phenotype Image Detail

Image

Caption

A: Hematoxylin and eosin staining of cross sections of testes from 12 dpp control (left) and Dnmt3l^tm1Bes/Dnmt3l^tm1Bes (right) mice. Germ cells do not appear to be entering meiosis in the absence of DNMT3L (right panel). B: Immunoperoxidase staining of germ cells with GCNA1 antibody (brown) in testis sections of 10 dpp (top) and 8 dpp (bottom) control and mutant mice. C: Graphical representation of germ cell counts in control and mutant mouse testes. A difference in germ cell count is observed as early as 6 dpp in absence of DNMT3L. By 10 dpp, mutant males have approximately a quarter of the number of germ cells their control littermates have. For each time point, GCNA-1 positive germ cells were count per 2000 Sertoli cells; two to three males were examined per genotype. Results are presented as means +/- SEM; mean counts for individual males are presented as dots when only two mice were analyzed.

Copyright

This image is from La Salle S, BMC Dev Biol 2007 Sep 18;7(1):104, an open-access article, licensee BioMed Central Ltd. J:128710

Associated Alleles

Symbol	Name
Dnmt3l^tm1Bes	DNA methyltransferase 3-like; targeted mutation 1, Timothy Bestor

Associated Genotypes

Allelic Composition	Genetic Background
Dnmt3l^tm1Bes/Dnmt3l^tm1Bes	involves: 129S6/SvEvTac