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Gene Expression Data
Assay Details
Assay
Reference: J:340701 Lee SJ, et al., Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway. Cell Death Differ. 2021 May;28(5):1441-1454
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8403135
Gene symbol: Mast4
Gene name: microtubule associated serine/threonine kinase family member 4
Antibody: anti-MAST4 (BS5791)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1E: postnatal day 1 (more )
Note: Double labeled: green - Mast4; red - Sox9 (assay MGI:8403136).
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Present Regionally restricted 1E , 1E inset Expression in cytoplasm of Sox9-expressing Sertoli cells.
Specimen 1F: postnatal day 1 (more )
Note: Double labeled: green - Mast4; red - Amh (assay MGI:8403138).
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Present Regionally restricted 1F , 1F inset Expression in Sertoli cells co-localized with Amh expression.
Specimen 1G: postnatal day 1 (more )
Note: Double labeled: green - Mast4; red - Ddx4 (assay MGI:8403140).
Structure Level Pattern Image Note
TS27: germ cell of testis Absent 1G inset , 1G Expression not -co-localized with Ddx4 expressing germ cells.
Specimen 1I: postnatal week 3 (more )
Note: Double labeled: green - Mast4; red - Sox9 (assay MGI:8403136).
Structure Cell Type Level Pattern Image Note
TS28: Leydig cell Leydig cell Present 1I inset , 1I Expression shifted to Leydig cells.
Specimen 1J: postnatal week 3 (more )
Note: Double labeled: green - Mast4; red - Hsd3b1 (assay MGI:8403141).
Structure Cell Type Level Pattern Image Note
TS28: Leydig cell Leydig cell Present 1J inset , 1J Expression in Leydig cells co-localized with Hsd3b1 expression.
Specimen 1K: postnatal week 3 (more )
Note: Double labeled: green - Mast4; red - Ddx4 (assay MGI:8403140).
Structure Level Pattern Image Note
TS28: spermatogonium Absent 1K inset , 1K Expression not co-localized with Ddx4 expression in spermatogonia.
Specimen 1N: postnatal week 6 (more )
Note: Double labeled: green - Mast4; red - Sox9 (assay MGI:8403136).
Structure Level Pattern Image Note
TS28: spermatogonium Present 1N , 1N inset
Specimen 1O: postnatal week 6; Mast4em1Sjkm/Mast4em1Sjkm (more )
Note: Double labeled: green - Mast4; red - Sox9 (assay MGI:8403136).
Structure Level Pattern Image Note
TS28: seminiferous tubule Absent 1O , 1O inset
Specimen S1B: embryonic day 15.5 (more )
Note: Double labeled: green - Mast4; red - Sox9 (assay MGI:8403136).
Structure Cell Type Level Pattern Image Note
TS23: Sertoli cell Sertoli cell Present Regionally restricted S1B inset , S1B Expression in cytoplasm of Sox9-expressing Sertoli cells.
Specimen S1C: embryonic day 15.5 (more )
Note: Double labeled: green - Mast4; red - Amh (assay MGI:8403138).
Structure Cell Type Level Pattern Image Note
TS23: Sertoli cell Sertoli cell Present Regionally restricted S1C inset , S1C Expression co-localized with Amh expression in cytoplasm of Sertoli cells from E15.5 to PN 1 W.
Specimen S1D: embryonic day 15.5 (more )
Note: Double labeled: green - Mast4; red - Ddx4 (assay MGI:8403140).
Structure Level Pattern Image Note
TS23: germ cell of testis Absent S1D , S1D inset Expression not co-localized with Ddx4-expressing spermatogonia and spermatocytes.
Specimen S1F: postnatal week 1 (more )
Note: Double labeled: green - Mast4; red - Sox9 (assay MGI:8403136).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present Regionally restricted S1F , S1F inset Expression in cytoplasm of Sox9-expressing Sertoli cells.
Specimen S1G: postnatal week 1 (more )
Note: Double labeled: green - Mast4; red - Amh (assay MGI:8403138).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present Regionally restricted S1G inset , S1G Expression co-localized with Amh expression in cytoplasm of Sertoli cells from E15.5 to PN 1 W.
Specimen S1H: postnatal week 1 (more )
Note: Double labeled: green - Mast4; red - Ddx4 (assay MGI:8403140).
Structure Level Pattern Image Note
TS28: spermatocyte Absent S1H , S1H inset Expression not co-localized with Ddx4-expressing spermatogonia and spermatocytes.
TS28: spermatogonium Absent S1H , S1H inset Expression not co-localized with Ddx4-expressing spermatogonia and spermatocytes.
Specimen S1J: postnatal week 13 (more )
Note: Double labeled: green - Mast4; red - Sox9 (assay MGI:8403136).
Structure Cell Type Level Pattern Image Note
TS28: elongated spermatid Present S1J inset , S1J Expression in pubertal elongated spermatids and spermatozoa.
TS28: spermatozoon Present S1J inset , S1J Expression in pubertal elongated spermatids and spermatozoa.
TS28: Leydig cell Leydig cell Present S1J , S1J inset
Specimen S1K: postnatal week 13 (more )
Note: Double labeled: green - Mast4; red - Hsd3b1 (assay MGI:8403141).
Structure Level Pattern Image Note
TS28: elongated spermatid Present S1K , S1K inset Expression in pubertal elongated spermatids and spermatozoa.
TS28: spermatozoon Present S1K , S1K inset Expression in pubertal elongated spermatids and spermatozoa.
TS28: Leydig cell Present S1K , S1K inset Expression co-expressed with Hsd3b1 expression in Leydig cells.
Specimen S1L: postnatal week 13 (more )
Note: Double labeled: green - Mast4; red - Ddx4 (assay MGI:8403140).
Structure Cell Type Level Pattern Image Note
TS28: spermatocyte Absent S1L , S1L inset Expression not co-localized with Ddx4-expressing spermatogonia and spermatocytes.
TS28: spermatogonium Absent S1L , S1L inset Expression not co-localized with Ddx4-expressing spermatogonia and spermatocytes.
TS28: elongated spermatid Present S1L inset , S1L Expression in pubertal elongated spermatids and spermatozoa.
TS28: spermatozoon Present S1L inset , S1L Expression in pubertal elongated spermatids and spermatozoa.
TS28: Leydig cell Leydig cell Present S1L inset , S1L
Specimen S5G: postnatal week 6 (more )
Structure Level Pattern Image Note
TS28: Leydig cell Present S5G inset , S5G
Specimen S5H: postnatal month 12 (more )
Structure Level Pattern Image Note
TS28: Leydig cell Present S5H , S5H inset
TS28: seminiferous tubule Absent S5H inset , S5H No expression inside seminiferous tubules.
Specimen S5I: postnatal month 20 (more )
Structure Cell Type Level Pattern Image Note
TS28: Leydig cell Leydig cell Present S5I , S5I inset
TS28: seminiferous tubule Absent S5I inset , S5I No expression in Sertoli cell-only (SCO) tubules.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
MGI 6.29 		The Jackson Laboratory