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Gene Expression Data
Assay Details
Assay
Reference: J:340701 Lee SJ, et al., Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway. Cell Death Differ. 2021 May;28(5):1441-1454
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8403136
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-SOX9 (14-9765-80)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1E: postnatal day 1 (more )
Note: Double labeled: red - Sox9; green - Mast4 (assay MGI:8403135).
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Present Regionally restricted 1E inset , 1E Expression in nuclei of Sertoli cells.

Specimen 1I: postnatal week 3 (more )
Note: Double labeled: red - Sox9; green - Mast4 (assay MGI:8403135).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 1I inset , 1I Continuous expression in Sertoli cells.

Specimen 1N: postnatal week 6 (more )
Note: Double labeled: red - Sox9; green - Mast4 (assay MGI:8403135).
Structure Level Pattern Image Note
TS28: Sertoli cell Present Regionally restricted 1N , 1N inset Expression in nuclei of Sertoli cells.

Specimen 1O: postnatal week 6; Mast4em1Sjkm/Mast4em1Sjkm (more )
Note: Double labeled: red - Sox9; green - Mast4 (assay MGI:8403135).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 1O inset , 1O Increased number of expressing cells in Sertoli cell-only (SCO) tubules.

Specimen S1B: embryonic day 15.5 (more )
Note: Double labeled: red - Sox9; green - Mast4 (assay MGI:8403135).
Structure Cell Type Level Pattern Image Note
TS23: Sertoli cell Sertoli cell Present S1B , S1B inset

Specimen S1F: postnatal week 1 (more )
Note: Double labeled: red - Sox9; green - Mast4 (assay MGI:8403135).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present S1F , S1F inset

Specimen S1J: postnatal week 13 (more )
Note: Double labeled: red - Sox9; green - Mast4 (assay MGI:8403135).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present S1J inset , S1J

Specimen 2D: postnatal week 6 (more )
Note: Double labeled: red - Sox9; green - Etv5 (assay MGI:8403143).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 2D , 2D inset Expression in Sertoli cells co-localized with Etv5 expression.

Specimen 2E: postnatal week 6; Mast4em1Sjkm/Mast4em1Sjkm (more )
Note: Double labeled: red - Sox9; green - Etv5 (assay MGI:8403143).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 2E , 2E inset


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/06/2026
MGI 6.29
The Jackson Laboratory