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Gene Expression Data
Assay Details
Assay
Reference: J:340701 Lee SJ, et al., Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway. Cell Death Differ. 2021 May;28(5):1441-1454
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8403143
Gene symbol: Etv5
Gene name: ets variant 5
Antibody: anti-ETV5 (ab102010)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2D: postnatal week 6 (more )
Note: Double labeled: green - Etv5; red - Sox9 (assay MGI:8403136).
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 2D inset , 2D Expression in Sertoli cells co-localized with Sox9 expression.
TS28: elongated spermatid Present 2D inset , 2D Expression in several elongated spermatids.

Specimen 2E: postnatal week 6; Mast4em1Sjkm/Mast4em1Sjkm (more )
Note: Double labeled: green - Etv5; red - Sox9 (assay MGI:8403136).
Structure Level Pattern Image Note
TS28: seminiferous tubule Weak Regionally restricted 2E , 2E inset Expression decreased compared to wild type. rarely observed even in Sertoli cell only (SCO) tubules.

Specimen S5P: postnatal week 6 (more )
Note: Double labeled: green - Etv5; blue - TO-PRO-3.
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present S5P inset , S5P

Specimen S5Q: postnatal month 12 (more )
Note: Double labeled: green - Etv5; blue - TO-PRO-3.
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Weak Regionally restricted S5Q inset , S5Q Expression decreased in Sertoli cells in tubules with depleted germ cells.
TS28: seminiferous tubule Sertoli cell Weak Regionally restricted S5Q inset , S5Q Expression decreased in Sertoli cells in tubules with depleted germ cells.

Specimen S5R: postnatal month 20 (more )
Note: Double labeled: green - Etv5; blue - TO-PRO-3.
Structure Level Pattern Image Note
TS28: seminiferous tubule Trace Regionally restricted S5R inset , S5R Expression not observed in Sertoli cell-only (SCO) tubules.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/06/2026
MGI 6.29
The Jackson Laboratory