Gene Expression Data
Assay Details
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Assay
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Reference:
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J:239974
Lacomme M, et al., The LGN protein promotes planar proliferative divisions in the neocortex but apicobasal asymmetric terminal divisions in the retina. Development. 2016 Feb 15;143(4):575-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:8355610
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Gene symbol:
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Gpsm2
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Gene name:
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G-protein signalling modulator 2 (AGS3-like, C. elegans)
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Antibody:
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anti-GPSM2 (HPA007327)
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Detection system:
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Alexa Fluor
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Assay notes:
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Two Gpsm2 antibodies were described, but the one used in this assay was not detailed. Anti-LGN MGI:8355349 may have been used in this assay.
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Results
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Specimen
1G:
postnatal day 0
(more )
Note: Double labeled: red - Gpsm2; green - Tjp1 (assay MGI:8355611).
Specimen
1G:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Gpsm2; green - Tjp1 (assay MGI:8355611).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: neural retina |
retinal progenitor cell |
Present |
Regionally restricted |
1G
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In interphase retinal progenitor cells, expression around plasma membrane, enriched at apical domain.
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Specimen
1H/H':
postnatal day 0
(more )
Note: Double labeled: red - Gpsm2; green - Tjp1 (assay MGI:8355611).
Specimen
1H/H':
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Gpsm2; green - Tjp1 (assay MGI:8355611).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: neural retina |
retinal progenitor cell |
Present |
Regionally restricted |
1H'
,
1H
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In prophase retinal progenitor cells, expression localized to basolateral membrane, mostly concentrated at lateral poles, and excluded from apical domain.
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Specimen
1I/I':
postnatal day 0
(more )
Note: Double labeled: red - Gpsm2; green - Tjp1 (assay MGI:8355611).
Specimen
1I/I':
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Gpsm2; green - Tjp1 (assay MGI:8355611).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: neural retina |
retinal progenitor cell |
Present |
Regionally restricted |
1I
,
1I'
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In horizontal anaphase/telophase retinal progenitor cells, expression localized to basolateral membrane, and absent from apical domain.
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Specimen
1J/J':
postnatal day 0
(more )
Note: Double labeled: red - Gpsm2; green - Tjp1 (assay MGI:8355611).
Specimen
1J/J':
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Gpsm2; green - Tjp1 (assay MGI:8355611).
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