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Gene Expression Data
Assay Details
Assay
Reference: J:348078 Nie X, et al., Piezo1 and Piezo2 collectively regulate jawbone development. Development. 2024 May 1;151(9):dev202386
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8288998
Gene symbol: Alpl
Gene name: alkaline phosphatase, liver/bone/kidney
Antibody: anti-ALPL (7H11L3), 702454
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1B: embryonic day 13.5 (more )
Note: Double labeled: red - Alpl; green - Piezo1 (assay MGI:8288996).
Structure Cell Type Level Pattern Image Note
TS21: mandible preosteoblast Present 1B Alp , 1B merge Co-localization with Piezo1 in the mandibular arches.
Specimen 1D: embryonic day 14.5 (more )
Note: Double labeled: red - Alpl; green - Piezo1 (assay MGI:8288996).
Structure Level Pattern Image Note
TS22: mandible Present 1D Alp , 1D merge Co-localization with Piezo1 in the mandibular arches.
Specimen 1J: postnatal week 3; Piezo1tm2.1Apat/Piezo1tm2.1Apat (more )
Note: Double labeled: red - Alpl; green - Piezo1 (assay MGI:8288996).
Structure Cell Type Level Pattern Image Note
TS28: mandible alveolar process osteoblast Present 1J merge , 1J Alp
Specimen 1L: postnatal week 3; E2f1Tg(Wnt1-cre)2Sor/E2f1+, Piezo1tm2.1Apat/Piezo1tm2.1Apat (more )
Note: Conditional mutant. Double labeled: red - Alpl; green - Piezo1 (assay MGI:8288996).
Structure Cell Type Level Pattern Image Note
TS28: mandible alveolar process osteoblast Present 1L merge , 1L Alp
Specimen 3G: postnatal day 0; Piezo1tm2.1Apat/Piezo1tm2.1Apat, Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Structure Cell Type Level Pattern Image Note
TS27: mandible osteoblast Present 3G
Specimen 3H: postnatal day 0; E2f1Tg(Wnt1-cre)2Sor/E2f1+, Piezo1tm2.1Apat/Piezo1tm2.1Apat, Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: mandible osteoblast Present 3H Reduced number of osteogenic cells in dCKO mice.
Specimen 3K: postnatal day 0; Piezo1tm2.1Apat/Piezo1tm2.1Apat, Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Note: Double labeled: red - Alpl; green - Ctnnb1 (assay MGI:8289040).
Structure Cell Type Level Pattern Image Note
TS27: mandible osteoblast Present 3K
Specimen 3L: postnatal day 0; E2f1Tg(Wnt1-cre)2Sor/E2f1+, Piezo1tm2.1Apat/Piezo1tm2.1Apat, Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Note: Conditional mutant. Double labeled: red - Alpl; green - Ctnnb1 (assay MGI:8289040).
Structure Cell Type Level Pattern Image Note
TS27: mandible osteoblast Present 3L Reduced number of osteogenic cells in dCKO mice.
Specimen 4E: postnatal day 0; E2f1Tg(Wnt1-cre)2Sor/E2f1+, Piezo1tm2.1Apat/Piezo1tm2.1Apat, Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Note: Conditional mutant. Double labeled: red - Alpl; green - TUNEL.
Structure Cell Type Level Pattern Image Note
TS27: mandible osteoblast Present 4E Majority of apoptotic cells overlapped with Alpl staining, suggesting that cell death was primarily seen in the osteogenic cell lineage in dCKO mice.
Specimen S1A: embryonic day 12.5 (more )
Note: Double labeled: red - Alpl; green - Piezo1 (assay MGI:8288996).
Structure Cell Type Level Pattern Image Note
TS20: jaw bone preosteoblast Present Regionally restricted S1A Co-localization with Piezo1 in the mandibular arches.
Specimen S1B: embryonic day 13.5 (more )
Note: Double labeled: red - Alpl; green - Piezo1 (assay MGI:8288996).
Structure Cell Type Level Pattern Image Note
TS21: mandible preosteoblast Present S1B Co-localization with Piezo1 in the mandibular arches.
Specimen S1C: embryonic day 14.5 (more )
Note: Double labeled: red - Alpl; green - Piezo1 (assay MGI:8288996).
Structure Cell Type Level Pattern Image Note
TS22: mandible preosteoblast Present S1C Co-localization with Piezo1 in the mandibular arches.
Specimen S1D: postnatal day 0 (more )
Note: Double labeled: red - Alpl; green - Piezo1 (assay MGI:8288996).
Structure Cell Type Level Pattern Image Note
TS27: mandible osteoblast Present S1D Co-localization with Piezo1 in the mandibular arches.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/14/2026
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