Piezo1^tm2.1Apat
Targeted Allele Detail

Summary

• Symbol: Piezo1^tm2.1Apat
• Name: piezo-type mechanosensitive ion channel component 1; targeted mutation 2.1, Ardem Patapoutian
• MGI ID: MGI:5766345
• Synonyms: P1^f
• Gene: Piezo1 Location: Chr8:123208437-123278068 bp, - strand Genetic Position: Chr8, 71.31 cM
• Alliance: Piezo1^tm2.1Apat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229436
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. The FRT-lacZ-loxP-neo-FRT-loxP cassette is introduced into intron 19 followed by a loxP site placed in intron 23. Flp-mediated recombination excised the lacZ and neomycin features, leaving exons 20-23 flanked by loxP sites. (J:229436)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piezo1 Mutation: 87 strains or lines available

References

• Original: J:229436 Cahalan SM, et al., Piezo1 links mechanical forces to red blood cell volume. Elife. 2015;4
• All: 34 reference(s)