Antibody:	Anti-Ephrin A2 (L-20): sc-912
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Present		2a'
TS22: cerebral cortex ventricular layer	Present		2a'

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone		Present		2b'' , 2b'
TS24: cerebral cortex ventricular layer		Present		2b'' , 2b'
TS24: cortical plate	cerebral cortex neuron	Present		2b' , 2b''	Immunopositive cells in the cortical plate also expressed the neuronal marker Rbfox3 (NeuN).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: telencephalon	interneuron	Present	Regionally restricted	6a'' , 6a''' , 6a'	At E14.5, Gad1- & Gad2-immunopositive interneurons emerging from two transient telencephalic regions, the medial ganglionic eminence (MGE) and the preoptic area (POA) also express Efna2. Efna2-immunopositive cells were organized in a continuous stream extending from the POA to dorsolateral telencephalon, correlating with the superficial migratory stream.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	cortical interneuron	Present	Regionally restricted	6c' , 6b'' , 6c , 6b'	Dense Gad1- & Gad2-stained interneurons in the marginal zone were double-labeled with Efna2-positive cells at E18.5.
TS26: cerebral cortex ventricular zone	cortical interneuron	Present	Regionally restricted	6b' , 6b''	A dense population of Efna2-immunopositive cells was located in the ventricular zone.
TS26: cortical plate	cortical interneuron	Present	Scattered	6b' , 6b''