Antibody:	anti-NeuN (ABN78)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Two antibodies were listed; the one used in this assay was not specified. Either MGI:6887423 or MGI:1929615 was used.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1a' series 1	An abnormal patch of low immunostaining was observed in the mutant. The patches established sharp boundaries between normal and disrupted zones and extended through the neocortex with the loss of neurons becoming more severe towards the center of the patch, as illustrated on the series of adjacent sections.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1a' series 2	An abnormal patch of low immunostaining was observed in the mutant. The patches established sharp boundaries between normal and disrupted zones and extended through the neocortex with the loss of neurons becoming more severe towards the center of the patch, as illustrated on the series of adjacent sections.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1a' series 3	An abnormal patch of low immunostaining was observed in the mutant. The patches established sharp boundaries between normal and disrupted zones and extended through the neocortex with the loss of neurons becoming more severe towards the center of the patch, as illustrated on the series of adjacent sections.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1a' series 4	An abnormal patch of low immunostaining was observed in the mutant. The patches established sharp boundaries between normal and disrupted zones and extended through the neocortex with the loss of neurons becoming more severe towards the center of the patch, as illustrated on the series of adjacent sections.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1a' series 5	An abnormal patch of low immunostaining was observed in the mutant. The patches established sharp boundaries between normal and disrupted zones and extended through the neocortex with the loss of neurons becoming more severe towards the center of the patch, as illustrated on the series of adjacent sections.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1a' series 6	An abnormal patch of low immunostaining was observed in the mutant. The patches established sharp boundaries between normal and disrupted zones and extended through the neocortex with the loss of neurons becoming more severe towards the center of the patch, as illustrated on the series of adjacent sections.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1b'	The distribution of patches with low immunostaining density was highly variable and apparently random, with the disruption affecting all layers indiscriminately (layers 2/3).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1c'	The distribution of patches with low immunostaining density was highly variable and apparently random, with the disruption affecting all layers indiscriminately (layers 5).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1d inset	Co-labeling with Aldh1l1 indicated astrocytes were noticeably reduced within the regions lacking neurons, with only a few cells observed close to the border.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1d' inset	Co-labeling with Olig2 indicated oligodendrocytes were noticeably reduced within the regions lacking neurons, with only a few cells observed close to the border.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	1d'' inset	Co-labeling with Aif1 (Iba1) indicated microglia were uniformly distributed in the abnormal region.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present		1e

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present		1e'	Outside the perturbed regions, immunostaining showed that the distribution of neurons appeared normal, and the cell density was not significantly different from wild type.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cortical plate	cerebral cortex neuron	Present		2b''	Efna2 immunopositive cells in the cortical plate also expressed the neuronal marker Rbfox3 (NeuN).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	cerebral cortex neuron	Present	Non-Uniform	5a'	An abnormal patch of low immunostaining was observed in the mutant.