Gene Expression Data
Assay Details
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Assay
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Reference:
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J:290119
Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:8270442
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-TH (rabbit; P40101-150)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 647
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Assay notes:
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The brain was processed using iDISCO optical clearing protocol. Note: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
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Results
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Specimen
1a:
embryonic day 18.5
(more  )
Specimen
1a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain |
Present |
Regionally restricted |
1a
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Expression was detected in dopaminergic axon bundles, which extend rostrally through the hypothalamus and innervate the striatum.
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Specimen
1b:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Zfp503tm1.1Bpb/Zfp503tm1.1Bpb
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain |
Present |
Regionally restricted |
1b
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Dopaminergic axons are misguided in Nolz1-/- mutant embryos, a large proportion of immunolabelled axons cross the midline in the hypothalamus
and terminate rostral of the striatum in the mutant embryos.
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Specimen
1c:
embryonic day 18.5
(more )
Specimen
1c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain |
Present |
Regionally restricted |
1c
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Expression was detected in dopaminergic axon bundles, which extend rostrally through the hypothalamus and innervate the striatum.
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Specimen
1d:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Zfp503tm1.1Bpb/Zfp503tm1.1Bpb
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: brain |
Present |
Regionally restricted |
1d
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Dopaminergic axons are misguided in Nolz1-/- mutant embryos, a large proportion of immunolabelled axons cross the midline in the hypothalamus
and terminate rostral of the striatum in the mutant embryos.
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Analysis Tools
