Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:321503 Meuser M, et al., FGFR2 signaling enhances the SHH-BMP4 signaling axis in early ureter development. Development. 2022 Jan 1;149(1):dev200021
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8262796
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 555

Results

2C +/+;Fgfr1^fl/+;Fgfr2^fl/+ ACTA2 ©

Specimen 2C +/+;Fgfr1^fl/+;Fgfr2^fl/+: embryonic day 18.5; Fgfr1^tm5.1Sor/Fgfr1⁺, Fgfr2^tm1Dor/Fgfr2⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: ureteral smooth muscle layer	ureter smooth muscle cell	Present		2C +/+;Fgfr1^fl/+;Fgfr2^fl/+ ACTA2

2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/+ ACTA2 ©

Specimen 2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/+: embryonic day 18.5; Fgfr1^tm5.1Sor/Fgfr1⁺, Fgfr2^tm1Dor/Fgfr2⁺, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: ureteral smooth muscle layer	ureter smooth muscle cell	Present		2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/+ ACTA2

2C Pax2-cre/+;Fgfr1^fl/fl;Fgfr2^fl/+ ACTA2 ©

Specimen 2C Pax2-cre/+;Fgfr1^fl/fl;Fgfr2^fl/+: embryonic day 18.5; Fgfr1^tm5.1Sor/Fgfr1^tm5.1Sor, Fgfr2^tm1Dor/Fgfr2⁺, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: ureteral smooth muscle layer	ureter smooth muscle cell	Present		2C Pax2-cre/+;Fgfr1^fl/fl;Fgfr2^fl/+ ACTA2

2C Pax2-cre/+;Fgfr1^fl/fl;Fgfr2^fl/fl ACTA2 ©

Specimen 2C Pax2-cre/+;Fgfr1^fl/fl;Fgfr2^fl/fl: embryonic day 18.5; Fgfr1^tm5.1Sor/Fgfr1^tm5.1Sor, Fgfr2^tm1Dor/Fgfr2^tm1Dor, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: ureteral smooth muscle layer	ureter smooth muscle cell	Present		2C Pax2-cre/+;Fgfr1^fl/fl;Fgfr2^fl/fl ACTA2

2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/fl ACTA2 left ©

Specimen 2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/fl left: embryonic day 18.5; Fgfr1^tm5.1Sor/Fgfr1⁺, Fgfr2^tm1Dor/Fgfr2^tm1Dor, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: ureteral smooth muscle layer	ureter smooth muscle cell	Present		2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/fl ACTA2 left

2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/fl ACTA2 right ©

Specimen 2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/fl right: embryonic day 18.5; Fgfr1^tm5.1Sor/Fgfr1⁺, Fgfr2^tm1Dor/Fgfr2^tm1Dor, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant. Undilated ureter.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: ureteral smooth muscle layer	ureter smooth muscle cell	Present		2C Pax2-cre/+;Fgfr1^fl/+;Fgfr2^fl/fl ACTA2 right

3B control E12.5 ACTA2 ©

Specimen 3B E12.5 control: embryonic day 12.5; Fgfr1^tm5.1Sor/?, Fgfr2^tm1Dor/? (more

)
Note: Cre-negative littermates were used as controls.

Structure	Level	Pattern	Image	Note
TS20: ureter	Ambiguous		3B control E12.5 ACTA2

3B Fgfr2cKO E12.5 ACTA2 ©

Specimen 3B E12.5 Fgfr2cKO: embryonic day 12.5; Fgfr1^tm5.1Sor/Fgfr1⁺, Fgfr2^tm1Dor/Fgfr2^tm1Dor, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: ureter	Ambiguous		3B Fgfr2cKO E12.5 ACTA2

3B control E14.5 ACTA2 ©

Specimen 3B E14.5 control: embryonic day 14.5; Fgfr1^tm5.1Sor/?, Fgfr2^tm1Dor/? (more

)
Note: Cre-negative littermates were used as controls.

Structure	Level	Pattern	Image	Note
TS22: ureter	Ambiguous		3B control E14.5 ACTA2

3B Fgfr2cKO E14.5 ACTA2 ©

Specimen 3B E14.5 Fgfr2cKO: embryonic day 14.5; Fgfr1^tm5.1Sor/Fgfr1⁺, Fgfr2^tm1Dor/Fgfr2^tm1Dor, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: ureter	Ambiguous		3B Fgfr2cKO E14.5 ACTA2

Specimen 3B E15.5 control: embryonic day 15.5; Fgfr1^tm5.1Sor/?, Fgfr2^tm1Dor/? (more

)
Note: Cre-negative littermates were used as controls.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: ureteral smooth muscle layer	ureter smooth muscle cell	Present		3B control E15.5 ACTA2

Specimen 3B E15.5 Fgfr2cKO: embryonic day 15.5; Fgfr1^tm5.1Sor/Fgfr1⁺, Fgfr2^tm1Dor/Fgfr2^tm1Dor, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: ureteral smooth muscle layer	ureter smooth muscle cell	Ambiguous		3B Fgfr2cKO E15.5 ACTA2

Specimen 3B E16.5 control: embryonic day 16.5; Fgfr1^tm5.1Sor/?, Fgfr2^tm1Dor/? (more

)
Note: Cre-negative littermates were used as controls.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ureteral smooth muscle layer	ureter smooth muscle cell	Present		3B control E16.5 ACTA2

Specimen 3B E16.5 Fgfr2cKO: embryonic day 16.5; Fgfr1^tm5.1Sor/Fgfr1⁺, Fgfr2^tm1Dor/Fgfr2^tm1Dor, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: ureteral smooth muscle layer	ureter smooth muscle cell	Present		3B Fgfr2cKO E16.5 ACTA2	Expression was delayed by 1 day.

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