Otx2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:372845 Napoli FR, et al., Microphthalmia and Disrupted Retinal Development Due to a LacZ Knock-in/Knock-Out Allele at the Vsx2 Locus. Eye Brain. 2024;16:115-131
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8257016
Gene symbol:	Otx2
Gene name:	orthodenticle homeobox 2

Antibody:

Anti-Otx2 (AF1979)

Results

2D ©

Specimen 2D: embryonic day 10.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: retina epithelium	Present	Regionally restricted	2D	Expression in pigmented epithelium.

2J ©

Specimen 2J: embryonic day 10.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2^{tm1b(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS17: retina epithelium	Present	Regionally restricted	2J	Expression similar to that of control.

2P ©

Specimen 2P: embryonic day 11.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: retina	Present		2P

2V ©

Specimen 2V: embryonic day 11.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2^{tm1b(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS19: retina	Present	Regionally restricted	2V	Expression elevated in central retina.

4E ©

Specimen 4E: embryonic day 12.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS20: retina	Present		4E	Expression in photoreceptor precursors.

4K ©

Specimen 4K: embryonic day 12.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2^{tm1b(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	4K	Ectopic expression in peripheral retina at E12.5, most notably on ventral side.

Specimen +/Z not shown: embryonic day 13.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS21: retina	Present

Specimen Z/Z not shown: embryonic day 13.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2^{tm1b(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted		Expression in central retina, but cells were sparce compared to control.

4Q ©

Specimen 4Q: embryonic day 15.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS23: retina	Present		4Q

4W ©

Specimen 4W: embryonic day 15.5; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2^{tm1b(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	4W	Expression restricted to central retina.

7M ©

Specimen 7M: postnatal day 0; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS27: retina	Present		7M

7N ©

Specimen 7N: postnatal day 0; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2^{tm1b(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS27: retina	Present	Regionally restricted	7N	Expressed in a disorganized manner.

8M ©

Specimen 8M: postnatal day 10; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present		8M

8N ©

Specimen 8N: postnatal day 10; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2^{tm1b(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	8N	Expression indicates presence of organized epical epithelium.

9M ©

Specimen 9M: postnatal day 28; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present		9M

9N ©

Specimen 9N: postnatal day 28; Vsx2^{tm1b(EUCOMM)Wtsi}/Vsx2^{tm1b(EUCOMM)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	9N	Aberrantly proliferating cells interspersed with progenitors, differentiating neuronal cells, Muller glia, retinal ganglion cells, amacrine cells and photoreceptors.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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