Vsx2^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Vsx2^{tm1b(EUCOMM)Wtsi}
• Name: visual system homeobox 2; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:8256892
• Synonyms: Vsx2^Lacz
• Gene: Vsx2 Location: Chr12:84616602-84642231 bp, + strand Genetic Position: Chr12, 39.28 cM
• Alliance: Vsx2^{tm1b(EUCOMM)Wtsi} page
• IMPC: Vsx2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:372845
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details:

  The L1L2_Bact_P cassette was inserted at position 84622570 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 84623305. The critical exon(s) is/are thus flanked by loxP sites. Cre expression without flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:372845)

Expression

In Mice Carrying this Mutation:

135 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vsx2 Mutation: 29 strains or lines available

References

• Original: J:372845 Napoli FR, et al., Microphthalmia and Disrupted Retinal Development Due to a LacZ Knock-in/Knock-Out Allele at the Vsx2 Locus. Eye Brain. 2024;16:115-131
• All: 1 reference(s)