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Gene Expression Data
Assay Details
Assay
Reference: J:206319 Welcker JE, et al., Insm1 controls development of pituitary endocrine cells and requires a SNAG domain for function and for recruitment of histone-modifying factors. Development. 2013 Dec;140(24):4947-58
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:8238960
Gene symbol: Insm1
Gene name: insulinoma-associated 1
Reporter: lacZ
Antibody: anti-B-galactosidase (ab9361)
Results
Specimen 3A: embryonic day 17.5; Insm1tm1Cbm/Insm1+ (more )
Note: Triple labeled: green - Insm1 reporter; blue - Pecam1 (assay MGI:8238532); red - Sox2 (assay MGI:8238525).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis Present 3A' , 3A
TS25: pituitary gland endothelial cell Absent 3A , 3A'
Specimen 3B: embryonic day 17.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Triple labeled: green - Insm1 reporter; blue - Pecam1 (assay MGI:8238532); red - Sox2 (assay MGI:8238525).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis Present 3B , 3B' Insm1<lacZ> mutants had a marked increase in the number of Sox2+ cells, which co-expressed beta-galactosidase. Beta-galactosidase cells were packed more densely in the anterior lobe of the pituitary compared with control mice.
TS25: pituitary gland endothelial cell Absent 3B' , 3B No obvious change in distribution of endothelial cells was observed in Insm1 mutant mice.
Specimen 3C: embryonic day 17.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: green - Insm1 reporter; red - Sox9 (assay MGI:8238526).
Structure Level Pattern Image Note
TS25: adenohypophysis Present 3C , 3C'
Specimen 3D: embryonic day 17.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: green - Insm1 reporter; red - Sox9 (assay MGI:8238526).
Structure Level Pattern Image Note
TS25: adenohypophysis Present 3D' , 3D Insm1<lacZ> mutants had a marked increase in the number of Sox9+ cells, which co-expressed beta-galactosidase. Beta-galactosidase cells were packed more densely in the anterior lobe of the pituitary compared with control mice.
Specimen 3E: embryonic day 17.5; Insm1tm1Cbm/Insm1+ (more )
Note: Triple labeled: green - Insm1; red - S100; blue - ER/TR7.
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis Present 3E' , 3E
TS25: pituitary gland fibroblast Absent 3E' , 3E
TS25: pituitary gland folliculostellate cell Absent 3E' , 3E
Specimen 3F: embryonic day 17.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Triple labeled: green - Insm1; red - S100; blue - ER/TR7.
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis Present 3F' , 3F
TS25: pituitary gland fibroblast Absent 3F , 3F' No obvious change in distribution of fibroblast (ER/TR7+) and folliculostellate (S100+) cells was observed in Insm1 mutant mice.
TS25: pituitary gland folliculostellate cell Absent 3F , 3F' No obvious change in distribution of fibroblast (ER/TR7+) and folliculostellate (S100+) cells was observed in Insm1 mutant mice.
Specimen S2D: embryonic day 13.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1; green - Tunel.
Structure Level Pattern Image Note
TS21: pituitary gland Present S2D Double labeling with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining did not detect apoptosis in the pituitary at E13.5.
Specimen S2E: embryonic day 13.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1; green - Tunel.
Structure Level Pattern Image Note
TS21: pituitary gland Present S2E Double labeling with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining did not detect apoptosis in the pituitary at E13.5.
Specimen S2F: embryonic day 15.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1; green - Tunel.
Structure Level Pattern Image Note
TS23: pituitary gland Present S2F Double labeling with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining did not detect apoptosis in the pituitary at E15.5.
Specimen S2G: embryonic day 15.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1; green - Tunel.
Structure Level Pattern Image Note
TS23: pituitary gland Present S2G Double labeling with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining did not detect apoptosis in the pituitary at E15.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
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