Gene Expression Data
Assay Details
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Assay
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Reference:
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J:206319
Welcker JE, et al., Insm1 controls development of pituitary endocrine cells and requires a SNAG domain for function and for recruitment of histone-modifying factors. Development. 2013 Dec;140(24):4947-58
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:8238526
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Gene symbol:
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Sox9
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Gene name:
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SRY (sex determining region Y)-box 9
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Results
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Specimen
1C:
embryonic day 17.5
(more  )
Note: Triple labeled: red - Sox9; green - Insm1 (assay MGI:8238523); blue - Sox2 (assay MGI:8238525).
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sox9; green - Insm1 (assay MGI:8238523); blue - Sox2 (assay MGI:8238525).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: adenohypophysis |
Present |
Regionally restricted |
1C
,
1C'
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Expression was detected in endocrine progenitors of the pituitary. A few cells co-expressed Sox9 and Insm1.
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Specimen
3C:
embryonic day 17.5; Insm1tm1Cbm/Insm1 +
(more )
Note: Double labeled: red - Sox9; green - Insm1 reporter (assay MGI:8238960).
Specimen
3C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Insm1tm1Cbm/Insm1+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox9; green - Insm1 reporter (assay MGI:8238960).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: pituitary gland |
Present |
Regionally restricted |
3C
,
3C'
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Expression was detected in endocrine progenitors of the pituitary.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Insm1tm1Cbm/Insm1tm1Cbm
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox9; green - Insm1 reporter (assay MGI:8238960).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: adenohypophysis |
Present |
Regionally restricted |
3D'
,
3D
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A marked increase in the number of Sox9+ cells was observed in the mutant. The supernumerary Sox9+ cells co-expressed beta-galactosidase in Insm1<lacZ> mutants.
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Specimen
8D:
embryonic day 17.5; Insm1tm2.1Cbm/Insm1 +
(more )
Note: Double labeled: green - Sox9; red - Insm1 (assay MGI:8238523).
Specimen
8D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Insm1tm2.1Cbm/Insm1+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Sox9; red - Insm1 (assay MGI:8238523).
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Specimen
8E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Insm1tm1Cbm/Insm1tm2.1Cbm
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Sox9; red - Insm1 (assay MGI:8238523).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: adenohypophysis |
Present |
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8E
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A significant increase in the number of Sox9+, but not Insm1+ cells was observed in Insm1<deltaSNAG> mutants compared with control. The
proportion of Sox9+ cells co-expressing Insm1 was increased in Insm1<deltaSNAG> mutants compared with control mice.
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Specimen
S6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Insm1tm2.1Cbm/Insm1+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: adenohypophysis |
Present |
Regionally restricted |
S6D
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Expression was detected in endocrine progenitors of the pituitary.
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Specimen
S6E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Insm1tm1Cbm/Insm1tm2.1Cbm
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: adenohypophysis |
Present |
Regionally restricted |
S6E
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The number of Sox9+ cells was increased in the Insm1<lacZ/deltaSNAG> pituitary.
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Analysis Tools