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Gene Expression Data
Assay Details
Assay
Reference: J:206319 Welcker JE, et al., Insm1 controls development of pituitary endocrine cells and requires a SNAG domain for function and for recruitment of histone-modifying factors. Development. 2013 Dec;140(24):4947-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8238523
Gene symbol: Insm1
Gene name: insulinoma-associated 1
Antibody: anti-Insm1
Assay notes: Two antibodies were listed for this gene: MGI:8238412 and MGI:8238413; the one used in this assay was not specified.
Results
Specimen 1A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: future pituitary Present Regionally restricted 1A Expression was located in the most ventral part of the pituitary anlage. Staining was localized in the nucleus.
Specimen 1B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: pituitary gland Present Regionally restricted 1B At E13.5, expression has expanded and is detected below the progenitor zone.
Specimen 1C: embryonic day 17.5 (more )
Note: Triple labeled: green - Insm1; blue - Sox2 (assay MGI:8238525); red - Sox9 (assay MGI:8238526).
Structure Level Pattern Image Note
TS25: adenohypophysis Present Widespread 1C' , 1C By E17.5, expression is present in the entire anterior pituitary. A few cells co-expressed Sox2 and Insm1, or Sox9 and Insm1.
Specimen 1D: embryonic day 17.5 (more )
Note: Triple labeled: green - Insm1; red - Sox2 (assay MGI:8238525); blue - hormones.
Structure Level Pattern Image Note
TS25: adenohypophysis Present Widespread 1D' , 1D By E17.5, expression is present in the entire anterior pituitary. A few cells co-expressed Sox2 and Insm1. The majority of Insm1+ cells co-expressed hormones.
Specimen 1E: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - Gh (assay MGI:8238527).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis somatotroph Present 1E Insm1 was co-expressed with growth hormone.
Specimen 1F: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - Prl (assay MGI:8238528).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis mammotroph Present 1F Insm1 was co-expressed with prolactin.
Specimen 1G: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - Tshb (assay MGI:8238529).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis thyrotroph Present 1G Insm1 was co-expressed with thyroid-stimulating hormone.
Specimen 1H: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - Fshb (assay MGI:8238530).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis gonadtroph Present 1H Insm1 was co-expressed with follicle-stimulating hormone.
Specimen 1I: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - Pomc (assay MGI:8238531).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis corticotroph Present 1I Insm1 was co-expressed with adrenocorticotrope hormone.
Specimen 1J: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - Pomc (assay MGI:8238911).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis melanocyte stimulating hormone secreting cell Present 1J Insm1 was co-expressed with melanocyte-stimulating hormone.
Specimen 1K: embryonic day 17.5 (more )
Note: Triple labeled: green - Insm1; red - Mki67 (assay MGI:8238907); blue - hormones.
Structure Level Pattern Image Note
TS25: adenohypophysis Present Regionally restricted 1K' , 1K Insm1+ cells only very rarely co-expressed Ki67 at E17.5, indicating that most Insm1+ cells have exited the cell cycle.
Specimen 1L: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - ER/TR7.
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis fibroblast Absent 1L Insm1 was not co-expressed with ER/TR7, a marker for fibroblasts.
Specimen 1M: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - S100.
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis folliculostellate cell Absent 1M Insm1 was not co-expressed with S100, a marker for folliculostellate cells.
Specimen 1N: embryonic day 17.5 (more )
Note: Double labeled: green - Insm1; red - Pecam1 (assay MGI:8238532).
Structure Cell Type Level Pattern Image Note
TS25: adenohypophysis endothelial cell Absent 1N Insm1 was not co-expressed with Pecam1, a marker for endothelial cells.
Specimen 8A: embryonic day 17.5; Insm1tm2.1Cbm/Insm1+ (more )
Note: Double labeled: green - Insm1; red - Sox2 (assay MGI:8238525).
Structure Level Pattern Image Note
TS25: adenohypophysis Present 8A
Specimen 8B: embryonic day 17.5; Insm1tm1Cbm/Insm1tm2.1Cbm (more )
Note: Double labeled: green - Insm1; red - Sox2 (assay MGI:8238525).
Structure Level Pattern Image Note
TS25: adenohypophysis Present 8B A significant increase in the number of Sox2+, but not Insm1+ cells was observed in Insm1<deltaSNAG> mutants compared with control. The proportion of Sox2+ cells co-expressing Insm1 was increased in Insm1<deltaSNAG> mutants compared with control mice.
Specimen 8D: embryonic day 17.5; Insm1tm2.1Cbm/Insm1+ (more )
Note: Double labeled: green - Insm1; red - Sox9 (assay MGI:8238526).
Structure Level Pattern Image Note
TS25: adenohypophysis Present 8D
Specimen 8E: embryonic day 17.5; Insm1tm1Cbm/Insm1tm2.1Cbm (more )
Note: Double labeled: green - Insm1; red - Sox9 (assay MGI:8238526).
Structure Level Pattern Image Note
TS25: adenohypophysis Present 8E A significant increase in the number of Sox9+, but not Insm1+ cells was observed in Insm1<deltaSNAG> mutants compared with control. The proportion of Sox9+ cells co-expressing Insm1 was increased in Insm1<deltaSNAG> mutants compared with control mice.
Specimen 8G: embryonic day 17.5; Insm1tm2.1Cbm/Insm1+ (more )
Note: Double labeled: brown - Insm1; blue - Myl1 (assay MGI:8238987).
Structure Level Pattern Image Note
TS25: adenohypophysis Present 8G
Specimen 8H: embryonic day 17.5; Insm1tm1Cbm/Insm1tm2.1Cbm (more )
Note: Double labeled: brown - Insm1; blue - Myl1 (assay MGI:8238987).
Structure Level Pattern Image Note
TS25: adenohypophysis Present 8H A subpopulation of cells expressing the truncated Insm1 protein co-express Myl1.
Specimen S6G: embryonic day 17.5; Insm1tm2.1Cbm/Insm1+ (more )
Structure Level Pattern Image Note
TS25: adenohypophysis Present S6G
Specimen S6H: embryonic day 17.5; Insm1tm1Cbm/Insm1tm2.1Cbm (more )
Structure Level Pattern Image Note
TS25: adenohypophysis Present S6H The number of Insm1+ cells was not significantly changed in the Insm1<lacZ/deltaSNAG> pituitary.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
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