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Gene Expression Data
Assay Details
Assay
Reference: J:370213 Windley SP, et al., NEDD4 Promotes Sertoli Cell Proliferation and Adult Leydig Cell Differentiation in the Murine Testis. Endocrinology. 2025 Jul 8;166(9)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8233776
Gene symbol: Nedd4
Gene name: neural precursor cell expressed, developmentally down-regulated 4
Results
Specimen S1A NEDD4 Control: embryonic day 11.5; Nedd4tm3.1Bros/? (more )
Note: Double labeled: green - Nedd4; magenta - Ddx4 (assay MGI:8233768).
Structure Level Pattern Image Note
TS19: gonad Present Ubiquitous S1A Control Ubiquitously expressed throughout gonad and surrounding tissues.
Specimen S1A NEDD4 Cre:flox/flox: embryonic day 11.5; Nedd4tm3.1Bros/Nedd4tm3.1Bros, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nedd4; magenta - Ddx4 (assay MGI:8233768).
Structure Level Pattern Image Note
TS19: gonad Weak Regionally restricted S1A Cre:flox/flox Reduced expression in most, although still observed in some, somatic cells.
TS19: coelomic epithelium of gonad Present S1A Cre:flox/flox Expression detectable in coelomic epithelial cells.
Specimen S1C NEDD4 Control: embryonic day 14.5; Nedd4tm3.1Bros/Nedd4tm3.1Bros, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nedd4; magenta - Sox9 (assay MGI:8233771).
Structure Level Pattern Image Note
TS22: testis Present Widespread S1C Control Widely expressed in control gonads.
Specimen S1C NEDD4 Cre:flox/flox: embryonic day 14.5; Nedd4tm3.1Bros/? (more )
Note: Double labeled: green - Nedd4; magenta - Sox9 (assay MGI:8233771).
Structure Cell Type Level Pattern Image Note
TS22: testis Weak Regionally restricted S1C Cre:flox/flox Expression at a much lower level compared to control.
TS22: Leydig cell Leydig cell Absent S1C Cre:flox/flox Expression absent in Sertoli cells and Leydig cells.
TS22: Leydig cell Sertoli cell Absent S1C Cre:flox/flox Expression absent in Sertoli cells and Leydig cells.
TS22: Sertoli cell Leydig cell Absent S1C Cre:flox/flox Expression absent in Sertoli cells and Leydig cells.
TS22: Sertoli cell Sertoli cell Absent S1C Cre:flox/flox Expression absent in Sertoli cells and Leydig cells.
TS22: germ cell of testis Present Regionally restricted S1C Cre:flox/flox Expression in germ cells within testis cords.
TS22: coelomic epithelium of testis Present S1C Cre:flox/flox Persistent expression within coelomic epithelium.
Specimen S2A NEDD4 Control: embryonic day 14.5; Nedd4tm3.1Bros/? (more )
Note: Double labeled: green - Nedd4; magenta - Nr5a1 (assay MGI:8233784).
Structure Cell Type Level Pattern Image Note
TS22: adrenal cortex cortical cell of adrenal gland Strong Regionally restricted S2A Control NEDD4 , S2A Control Merge
TS22: adrenal medulla chromaffin cell of adrenal gland Absent S2A Control NEDD4 , S2A Control Merge Expression absent from chromaffin cells of adrenal medulla.
Specimen S2A NEDD4 Cre:flox/flox: embryonic day 14.5; Nedd4tm3.1Bros/Nedd4tm3.1Bros, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: green - Nedd4; magenta - Nr5a1 (assay MGI:8233784).
Structure Cell Type Level Pattern Image Note
TS22: adrenal cortex cortical cell of adrenal gland Absent S2A Cre:flox/flox NEDD4 , S2A Cre:flox/flox Merge Expression absent from Nr5a1-expressing cortical cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/24/2026
MGI 6.24 		The Jackson Laboratory