Antibody:	anti-peripherin (AB1530)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS23: pelvic ganglion	Present		4b

Structure	Level	Pattern	Image	Note
TS28: pelvic ganglion	Present		5a +/+
TS28: nerve of bladder fundus	Present		5c +/+ , 5a +/+ , 5b +/+
TS28: nerve of bladder neck	Present		5b +/+ , 5a +/+

Structure	Level	Pattern	Image	Note
TS28: nerve of bladder neck	Present		5a -/- , 5b -/-	Large nerve bundles in the bladder base continuous with the pelvic ganglia appeared to be less compactly bundled in the mutant.
TS28: nerve of bladder fundus	Present		5b -/- , 5c -/- , 5a -/-	Increased density of smaller nerves in the bladder body, compared with the wild-type.
TS28: pelvic ganglion	Present		5a -/-

Structure	Level	Pattern	Image	Note
TS28: nerve of bladder fundus	Present		6e +/+

Structure	Level	Pattern	Image	Note
TS28: nerve of bladder fundus	Present		6e -/-	Bundles of positive nerves in bladder body appeared more prominent in Hpse2 mutants than in control bladders.

Structure	Level	Pattern	Image	Note
TS28: pelvic ganglion	Present		7a WT Peripherin
TS28: nerve of bladder neck	Present		7a WT Peripherin	Immunostaining revealed ganglia flanking the outflow tract, with prominent nerve bundles between them, lying in the dorsal side of the outflow.

Structure	Level	Pattern	Image	Note
TS28: pelvic ganglion	Present		7a Lrig2-/- Peripherin
TS28: nerve of bladder neck	Present		7a Lrig2-/- Peripherin	A spectrum of aberrations was observed, from disorganized to almost absent positive nerves.

Structure	Level	Pattern	Image	Note
TS28: pelvic ganglion	Present		7a Hpse2-/- Peripherin
TS28: nerve of bladder neck	Present		7a Hpse2-/- Peripherin	A spectrum of aberrations was observed, from disorganized to almost absent positive nerves.